A new model of transient cerebral ischemia in neonatal rats

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
N MitsufujiT Sawada

Abstract

A new model of transient cerebral ischemia in 10-day- old rats is described. Under microscopic guidance, the right external and internal carotid arteries were electrically coagulated. A solid 0.47 mm diameter nylon thread was inserted into the right common carotid artery toward the ascending aorta up to 10-12 mm from the upper edge of the sternomastoid muscle (preischemic rats). A 60-min cerebral ischemia was induced by clamping the left external and internal carotid arteries (ischemic rats), followed by 3-h recirculation. 31P magnetic resonance (MRS) spectroscopic studies revealed that severe intracellular acidosis occurred and ATP disappeared completely for a least the last 20 min of ischemia. Cerebral blood flow (CBF), measured by the hydrogen clearance technique, decreased to approximately 11% of the preischemic level in the frontal cortex soon after the induction of ischemia. On resuscitation, ATP recovered completely and the preischemic intracellular pH level was restored within 180 min. CBF has recovered to approximately 30% of the preischemic level at 5 min after resuscitation. The CBF recovery was not compete even at 180 min after resuscitation. With this model, the effects of pure ischemia without hypoxia on the neona...Continue Reading

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Citations

Feb 5, 2008·Ultrasound in Medicine & Biology·Philippe BonninSylvain Renolleau
Jul 9, 2013·Experimental Neurology·C Charriaut-MarlangueS Renolleau
May 9, 2013·Experimental Neurology·Masahiro TsujiTomohiro Matsuyama
May 29, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hans-Jörg BuschKonstantin-Alexander Hossmann
Dec 27, 2017·International Journal of Molecular Sciences·Christiane Charriaut-MarlangueOlivier Baud
Jul 11, 1998·Stroke; a Journal of Cerebral Circulation·S RenolleauC Charriaut-Marlangue
Aug 23, 2018·Frontiers in Neurology·Christiane Charriaut-Marlangue, Olivier Baud

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