A new model to analyze metaphyseal bone healing in mice

The Journal of Surgical Research
T HistingM D Menger

Abstract

Despite the increasing clinical problems with metaphyseal fractures, most experimental studies investigate the healing of diaphyseal fractures. Although the mouse would be the preferable species to study the molecular and genetic aspects of metaphyseal fracture healing, a murine model does not exist yet. Using a special locking plate system, we herein introduce a new model, which allows the analysis of metaphyseal bone healing in mice. In 24 CD-1 mice the distal metaphysis of the femur was osteotomized. After stabilization with the locking plate, bone repair was analyzed radiologically, biomechanically, and histologically after 2 (n=12) and 5 wk (n=12). Additionally, the stiffness of the bone-implant construct was tested biomechanically ex vivo. The torsional stiffness of the bone-implant construct was low compared with nonfractured control femora (0.23 ± 0.1 Nmm/°versus 1.78 ± 0.15 Nmm/°, P<0.05). The cause of failure was a pullout of the distal screw. At 2 wk after stabilization, radiological analysis showed that most bones were partly bridged. At 5 wk, all bones showed radiological union. Accordingly, biomechanical analyses revealed a significantly higher torsional stiffness after 5 wk compared with that after 2 wk. Successf...Continue Reading

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Citations

Jul 1, 2016·Acta Orthopaedica·Olof H Sandberg, Per Aspenberg
May 28, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Olof SandbergPer Aspenberg
Jan 21, 2018·Calcified Tissue International·M KomrakovaS Sehmisch
Aug 25, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Melanie Haffner-LuntzerMichael Lehmicke

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