A new molecular biology approach in morphology: basic method and application of laser microdissection

Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan
T Tachikawa, T Irie

Abstract

Our understanding of the role played by specific genes in various diseases is advanced as elucidation of the human genome progresses. One important step in this process is profiling gene expression so as to understand the roles of specific genes and the role of the translated protein. Profiling of the gene should also include tissue generation and an explanation of the mechanism of the disease. A useful technique in achieving this goal is the laser microdissection (LMD) method, which can draw genetic information from tissues of individual patients. We describe herein our technique using a special film which we developed and laser microdissection by the Palm Co. With this LMD method, RNA was extracted from carcinoma tissue and genetic analysis was carried out. LMD is equivalent to pathological diagnosis, and it is necessary to do dissection for accurate diagnosis. It seems to make clear gene pathological diagnosis possible in the future.

Citations

Aug 5, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Naoki IkenagaMasao Tanaka
Jul 11, 2012·International Journal of Vascular Medicine·Ayami IkedaTsutomu Imaizumi
Feb 25, 2011·Annals of Surgical Oncology·Hiroshi SakaiMasao Tanaka
Apr 22, 2014·Annals of Surgical Oncology·Kohei NakataMasao Tanaka
Jan 20, 2007·International Journal of Cancer. Journal International Du Cancer·Kenoki OhuchidaMasao Tanaka
Mar 30, 2011·Cancer Science·Tadashi KayashimaMasao Tanaka
Dec 23, 2008·Molecular Cancer·Jun YuMasao Tanaka

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