A new molecular diagnostic tool for surveying and monitoring Triops cancriformis populations

PeerJ
Graham S SellersAfrica Gómez

Abstract

The tadpole shrimp, Triops cancriformis, is a freshwater crustacean listed as endangered in the UK and Europe living in ephemeral pools. Populations are threatened by habitat destruction due to land development for agriculture and increased urbanisation. Despite this, there is a lack of efficient methods for discovering and monitoring populations. Established macroinvertebrate monitoring methods, such as net sampling, are unsuitable given the organism's life history, that include long lived diapausing eggs, benthic habits and ephemerally active populations. Conventional hatching methods, such as sediment incubation, are both time consuming and potentially confounded by bet-hedging hatching strategies of diapausing eggs. Here we develop a new molecular diagnostic method to detect viable egg banks of T. cancriformis, and compare its performance to two conventional monitoring methods involving diapausing egg hatching. We apply this method to a collection of pond sediments from the Wildfowl & Wetlands Trust Caerlaverock National Nature Reserve, which holds one of the two remaining British populations of T. cancriformis. DNA barcoding of isolated eggs, using newly designed species-specific primers for a large region of mtDNA, was us...Continue Reading

References

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Datasets Mentioned

BETA
AB084514.1

Methods Mentioned

BETA
PCR
PCRs
electrophoresis

Software Mentioned

CodonCode Aligner
R R Core
Primer BLAST
Aliview
blmeco
lme4
HotShot
BLASTn
R

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