A new molecular marker for species-specific identification of Microsporum canis.

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Anita Ciesielska, Paweł Stączek

Abstract

Species identification of dermatophytes by conventional mycological methods based on macro- and microscopy analysis is time-consuming and has a lot of limitations such as slow fungal growth or low specificity. Thus, there is a need for the development of molecular methods that would provide reliable and prompt identification of this group of medically important fungi. The are many reports in the literature concerning PCR identification of dermatophyte species, but still, there are not many PCR assays for the separate detection of members of the genera Microsporum, especially Microsporum canis (zoophilic species) and Microsporum audouinii (anthropophilic species). The correct distinction of these species is important to determine the source of infection to implement the appropriate action to eliminate the path of infection transmission. In this paper, we present such a PCR-based method targeting velB gene that uses a set of two primers-Mc-VelB-F (5'-CTTCCCCACCCGCAACATC-3') and Mc-VelB-R (5'-TGTGGCTGCACCTGAGAGTGG-3'). The amplified fragment is specific due to the presence of (CAGCAC)8 microsatellite sequence only in the velB gene of M. canis. DNA from 153 fungal samples was used in PCR assay followed by electrophoretic analysis. ...Continue Reading

References

Feb 14, 2006·Journal of Dermatological Science·Anita DobrowolskaMagdalena Kozłowska
Mar 24, 2007·Bioinformatics·Triinu Koressaar, Maido Remm
Nov 6, 2009·Medical Mycology·Anna Brillowska-DabrowskaMaiken Cavling Arendrup
Jun 11, 2011·FEMS Microbiology Reviews·Ozgür Bayram, Gerhard H Braus
Sep 10, 2011·Journal of Medical Microbiology·Ali Rezaei-MatehkolaeiHossein Mirhendi
Jan 9, 2013·Medical Mycology·Anna Brillowska-DabrowskaMaiken Cavling Arendrup
Oct 21, 2016·Journal of the European Academy of Dermatology and Venereology : JEADV·N KobylakA Brillowska-Dąbrowska

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
Geneious
Primer
BLAST ( Basic Local Alignment Search Tool )

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