A new motif necessary and sufficient for stable localization of the delta2 glutamate receptors at postsynaptic spines.

The Journal of Biological Chemistry
Shinji MatsudaMichisuke Yuzaki

Abstract

The number of each subclass of ionotropic glutamate receptors (iGluRs) at the spines is differentially regulated either constitutively or in a neuronal activity-dependent manner. The delta2 glutamate receptor (GluRdelta2) is abundantly expressed at the spines of Purkinje cell dendrites and controls synaptic plasticity in the cerebellum. To obtain clues to the trafficking mechanism of the iGluRs, we expressed wild-type or mutant GluRdelta2 in cultured hippocampal and Purkinje neurons and analyzed their intracellular localization using immunocytochemical techniques. Quantitative analysis revealed that deletion of the 20 amino acids at the center of the C terminus (region E) significantly reduced the amount of GluRdelta2 protein at the spines in both types of neurons. This effect was partially antagonized by the inhibition of endocytosis by high dose sucrose treatment or coexpression of dominant negative dynamin. In addition, mutant GluRdelta2 lacking the E region (GluRdelta2DeltaE), but not wild-type GluRdelta2, was found to colocalize with the endosomal markers Rab4 and Rab7. Moreover, the antibody-feeding assay revealed that GluRdelta2DeltaE was internalized more rapidly than GluRdelta2wt. These results indicate that the E regi...Continue Reading

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Citations

Jun 4, 2008·Molecular Neurobiology·Sabine M Schmid, Michael Hollmann
Apr 22, 2008·Neuroscience·G MandolesiP Strata
Jun 29, 2011·Journal of Neuroscience Methods·Wolfgang WagnerJohn A Hammer
Apr 12, 2007·The European Journal of Neuroscience·Kazuhisa KohdaMichisuke Yuzaki

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