PMID: 6988671Jan 1, 1980Paper

A new mutation rpoD800, affecting the sigma subunit of E. coli RNA polymerase is allelic to two other sigma mutants

Molecular & General Genetics : MGG
H LiebkeR R Burgess

Abstract

We have characterized a new mutation rpoD800 affecting the sigma gene of E. coli. Upon tranfer to high temperature, a strain with the rpoD800 mutation ceases growth within 30 min. We find that this mutation renders sigma about 10-fold more thermolabile than the wild type sigma at 45 degrees C in vitro. We have compared the temperature profile for inactivation of wild type and mutant sigma and find that the mutant inactivates at a temperature about 9 degrees C lower than does the wild type. The chromosomal locus affected by rpoD800 is shown to be allelic to the locus affected by the spontaneous mutants ts285 and alt-1. All three mutations result in altered sigma and in altered growth at high temperature. We argue that the single locus affected is the structural gene for the sigma subunit of E. coli RNA polymerase.

References

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Citations

Jan 1, 1981·Molecular & General Genetics : MGG·Y N Zograff
Jan 1, 1983·Molecular & General Genetics : MGG·Y A OvchinnikovR B Khesin
Oct 1, 1979·Molecular & General Genetics : MGG·R R BurgessP A Lowe
Aug 12, 2010·Molecular Genetics and Genomics : MGG·Beata Nadratowska-WesołowskaGrzegorz Wegrzyn
Jul 29, 2016·Frontiers in Microbiology·Orlando SantillánGuillermo Dávila
May 23, 2019·Molecular Microbiology·Michael A LonettoMark J Buttner
Dec 1, 1995·Journal of Bacteriology·J Malakooti, B Ely
Sep 1, 1986·Microbiological Reviews·R H Doi, L F Wang
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Apr 20, 1989·Journal of Molecular Biology·T GardellaM M Susskind

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