A new nanoscale transdermal drug delivery system: oil body-linked oleosin-hEGF improves skin regeneration to accelerate wound healing

Journal of Nanobiotechnology
Weidong QiangHaiyan Li

Abstract

Epidermal growth factor (EGF) can promote cell proliferation as well as migration, which is feasible in tissue wound healing. Oil bodies have been exploited as an important platform to produce exogenous proteins. The exogenous proteins were expressed in oil bodies from plant seeds. The process can reduce purification steps, thereby significantly reducing the purification cost. Mostly, the diameter of oil body particle ranges between 1.0 and 1.5 µm in the safflower seeds, however, it reduces to 700-1000 nm in the transgenic safflower seeds. The significant reduction of particle size in transgenic seeds is extremely beneficial to skin absorption. The diameter of oil body in the transgenic safflower seeds was recorded in the range of 700-1000 nm. The smaller particle size improved their skin absorption. The expression level of oleosin-hEGF-hEGF in T3 transgenic seeds was highest at 69.32 mg/g of seeds. The oil body expressing oleosin-hEGF-hEGF had significant proliferative activity on NIH/3T3 cells and improved skin regeneration thereby accelerating wound healing in rats. The wound coverage rate exceeded 98% after treatment for 14 days with oil body expressing oleosin-hEGF-hEGF, while the saline without EGF group and wild type oil...Continue Reading

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Citations

Nov 17, 2020·Protein Expression and Purification·Huifang GuoXingchun Gou
May 1, 2021·Vaccines·Jennifer Schwestka, Eva Stoger
May 3, 2021·Biochimica Et Biophysica Acta. Biomembranes·Jeffrey A JulienKerney Jebrell Glover
Oct 2, 2021·Proteins·Jeffrey A JulienKerney Jebrell Glover

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Methods Mentioned

BETA
transgenic
biopsy
electrophoresis
PCR

Software Mentioned

Quantity One
Image J
GraphPad Prism

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