A new non-synchronous preparative counter-current centrifuge-the next generation of dynamic extraction/chromatography devices with independent mixing and settling control, which offer a step change in efficiency

Journal of Chromatography. a
S N IgnatovaIan A Sutherland

Abstract

A new and significantly more robust design of non-synchronous coil planet centrifuge is introduced where the degree of mixing between two immiscible phases can be changed independently from the "g" field required to separate out the phases. A hypothesis that an optimum ratio between the speed of the bobbin and the speed of the rotor can be found to optimise the efficiency of the separation for a given force field is upheld for an intermediate polarity phase system. This paves the way for extensive further research to find the optimum non-synchronous conditions for a range of different phase systems that are desirable for the separation of large molecules, proteins and biologics but can tend to emulsify in the standard "J" type centrifuge systems currently available and routinely in use for aqueous organic phase systems. A step change of up to 30% in resolution and 90% in plate efficiency is demonstrated.

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Citations

Jul 16, 2015·Journal of Natural Products·J Brent FriesenGuido F Pauli
Jul 12, 2011·Journal of Chromatography. a·Remco N A M van den Heuvel, Carola S König
Nov 15, 2018·Journal of Separation Science·Kai-Jun QuanDuo-Long Di
Aug 12, 2017·Topics in Current Chemistry·Leslie BrownKenneth R Seddon
Mar 15, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazufusa ShinomiyaYoichiro Ito

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