A new opioid peptide predicted from cloned cDNAs from skin of Pachymedusa dacnicolor and Agalychnis annae

FEBS Letters
Christian WechselbergerL Negri

Abstract

We have isolated a cDNA encoding a precursor of dermorphin from the skin of Pachymedusa dacnicolor. Besides four copies of this opioid peptide, the deduced sequence also contains the genetic information for a novel peptide Tyr-Ile-Phe-His-Leu-Met-Asp-NH2. This differs from Met-deltorphin by the presence of Ile at position 2. In a related precursor from the skin of Agalychnis annae, the sequence of this peptide is in the 3'-untranslated region of the cloned cDNA. From earlier results we predict that in skin peptides the second residue is D-allo-Ile. We have synthesized this and related peptides with different D-amino acids, and determined their delta agonist activity. The peptide with D-nor-Leu binds with high affinity to delta receptors, while that with D-allo-Ile is about 100 times less active.

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Citations

Mar 23, 2011·Amino Acids·Alexander JilekGünther Kreil
Nov 25, 2000·Peptides·L NegriR Lattanzi
Jun 19, 2009·The Journal of Biological Chemistry·Dewen YouRen Lai
Jul 11, 2006·Journal of Molecular Microbiology and Biotechnology·Dorjee G Tamang, Milton H Saier
Nov 22, 2018·Journal of Cellular and Molecular Medicine·Qilin LongYuxin Wu
Oct 2, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·David H MastJonathan V Sweedler
Mar 18, 1999·Progress in Neurobiology·L H LazarusS Salvadori

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