A new optical leaf-clip meter for simultaneous non-destructive assessment of leaf chlorophyll and epidermal flavonoids.

Physiologia Plantarum
Zoran G CerovicGwendal Latouche

Abstract

We have characterized a new commercial chlorophyll (Chl) and flavonoid (Flav) meter called Dualex 4 Scientific (Dx4). We compared this device to two other Chl meters, the SPAD-502 and the CCM-200. In addition, Dx4 was compared to the leaf-clip Dualex 3 that measures only epidermal Flav. Dx4 is factory-calibrated to provide a linear response to increasing leaf Chl content in units of µg cm(-2), as opposed to both SPAD-502 and CCM-200 that have a non-linear response to leaf Chl content. Our comparative calibration by Chl extraction confirmed these responses. It seems that the linear response of Dx4 derives from the use of 710 nm as the sampling wavelength for transmittance. The major advantage of Dx4 is its simultaneous assessment of Chl and Flav on the same leaf spot. This allows the generation of the nitrogen balance index (NBI) used for crop surveys and nitrogen nutrition management. The Dx4 leaf clip, that incorporates a GPS receiver, can be useful for non-destructive estimation of leaf Chl and Flav contents for ecophysiological research and ground truthing of remote sensing of vegetation. In this work, we also propose a consensus equation for the transformation of SPAD units into leaf Chl content, for general use.

References

Oct 10, 2002·Journal of Environmental Quality·Gregory A Carter, Bruce A Spiering
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May 1, 1971·Plant Physiology·J T Woolley
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Citations

Oct 17, 2013·Photosynthesis Research·Jan HlavinkaMartina Špundová
May 14, 2014·Journal of the Science of Food and Agriculture·María-Ángeles Del-Castillo-AlonsoEncarnación Núñez-Olivera
Mar 19, 2014·Plant, Cell & Environment·Christopher ParryBruce Bugbee
Aug 9, 2015·Environmental Pollution·Matthieu BagardDidier Le Thiec
Apr 9, 2015·Frontiers in Plant Science·Marie van Maarschalkerweerd, Søren Husted
Nov 9, 2016·Frontiers in Plant Science·Agata CieślaJan Sadowski
Mar 2, 2018·International Journal of Molecular Sciences·Hoang Chinh NguyenKai-Chieh Chang
Jun 16, 2017·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Elodie MerlierKamel Soudani
Aug 16, 2016·American Journal of Botany·Andrea E BerardiDouglas R Taylor
Nov 26, 2015·Science Advances·Jesse R LaskyGeoffrey P Morris
Jan 11, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yan YanPedro J Aphalo
May 16, 2019·Physiologia Plantarum·Anne-Claire TalhouëtPeter Streb
May 16, 2019·Journal of Integrative Plant Biology·Antonio MauceriFrancesco Sunseri
Dec 21, 2016·Frontiers in Plant Science·María L Pérez-BuenoMatilde Barón
Jul 1, 2018·Sensors·Francisco M PadillaRodney B Thompson
Mar 7, 2020·Frontiers in Plant Science·Saara Maria HartikainenThomas Matthew Robson
Jan 21, 2017·Frontiers in Plant Science·Alessandro VannozziMargherita Lucchin
Dec 14, 2018·Frontiers in Plant Science·Francisco M PadillaRodney B Thompson

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Software Mentioned

Dualex
excel
statistica
FORCE

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