A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)

Scientific Reports
Rujuta D MunjeShalini Prasad

Abstract

Successful commercialization of wearable diagnostic sensors necessitates stability in detection of analytes over prolonged and continuous exposure to sweat. Challenges are primarily in ensuring target disease specific small analytes (i.e. metabolites, proteins, etc.) stability in complex sweat buffer with varying pH levels and composition over time. We present a facile approach to address these challenges using RTILs with antibody functionalized sensors on nanoporous, flexible polymer membranes. Temporal studies were performed using both infrared spectroscopic, dynamic light scattering, and impedimetric spectroscopy to demonstrate stability in detection of analytes, Interleukin-6 (IL-6) and Cortisol, from human sweat in RTILs. Temporal stability in sensor performance was performed as follows: (a) detection of target analytes after 0, 24, 48, 96, and 168 hours post-antibody sensor functionalization; and (b) continuous detection of target analytes post-antibody sensor functionalization. Limit of detection of IL-6 in human sweat was 0.2 pg/mL for 0-24 hours and 2 pg/mL for 24-48 hours post-antibody sensor functionalization. Continuous detection of IL-6 over 0.2-200 pg/mL in human sweat was demonstrated for a period of 10 hours pos...Continue Reading

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Citations

Jan 4, 2018·Materials·Nicolai PopovPiotr Popov
Jun 2, 2018·Lab on a Chip·Anastasios EconomouMamas Prodromidis
Apr 24, 2020·Lab on a Chip·Sayali Upasham, Shalini Prasad
May 26, 2018·Future Science OA·Vikramshankar KamakotiShalini Prasad
Nov 15, 2018·Scientific Reports·Sayali UpashamShalini Prasad
Oct 19, 2017·Scientific Reports·David KinnamonShalini Prasad
Oct 15, 2019·Future Science OA·Paul RiceShalini Prasad
Feb 26, 2019·Nature Biotechnology·Jayoung KimJoseph Wang
Jan 28, 2021·Small·Ganganath S PereraSharath Sriram
Jan 9, 2021·Journal of Colloid and Interface Science·Michael BenjaminSellappan Senthilkumar
Mar 30, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Lyndon JonesMark Willcox
Oct 19, 2019·Analytical Chemistry·Juliane R SempionattoJoseph Wang
Oct 15, 2021·Nature Communications·Julia BrunmairChristopher Gerner
Feb 26, 2020·Digital Biomarkers·Noé Brasier, Jens Eckstein
Dec 1, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Jerod M RasmussenPathik D Wadhwa

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Methods Mentioned

BETA
biosensors
biosensor
X-ray
Dynamic Light Scattering
AFM
biosensing
NMR
electrophoretic light scattering

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