A new pathway for glucose-dependent insulinotropic polypeptide (GIP) receptor signaling: evidence for the involvement of phospholipase A2 in GIP-stimulated insulin secretion

The Journal of Biological Chemistry
J A EhsesC H McIntosh

Abstract

The hormone glucose-dependent insulinotropic polypeptide (GIP) is an important regulator of insulin secretion. GIP has been shown to increase adenylyl cyclase activity, elevate intracellular Ca(2+) levels, and stimulate a mitogen-activated protein kinase pathway in the pancreatic beta-cell. In the current study we demonstrate a role for arachidonic acid in GIP-mediated signal transduction. Static incubations revealed that both GIP (100 nm) and ATP (5 microm) significantly increased [(3)H]arachidonic acid ([(3)H]AA) efflux from transfected Chinese hamster ovary K1 cells expressing the GIP receptor (basal, 128 +/- 11 cpm/well; GIP, 212 +/- 32 cpm/well; ATP, 263 +/- 35 cpm/well; n = 4; p < 0.05). In addition, GIP receptors were shown for the first time to be capable of functionally coupling to AA production through Gbetagamma dimers in Chinese hamster ovary K1 cells. In a beta-cell model (betaTC-3), GIP was found to elicit [(3)H]AA release, independent of glucose, in a concentration-dependent manner (EC(50) value of 1.4 +/- 0.62 nm; n = 3). Although GIP did not potentiate insulin release under extracellular Ca(2+)-free conditions, it was still capable of elevating intracellular cAMP and stimulating [(3)H]AA release. Our data sugge...Continue Reading

References

Aug 1, 1975·The Journal of Clinical Endocrinology and Metabolism·J M FalkoE L Mazzaferri
Dec 1, 1992·Molecular and Cellular Endocrinology·M A WahlH P Ammon
Oct 1, 1992·The New England Journal of Medicine·A LacroixR Taillefer
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C L Jelsema, J Axelrod
Jan 1, 1986·Diabetologia·M NauckW Creutzfeldt
Aug 1, 1974·The Journal of Clinical Endocrinology and Metabolism·S CatalandE L Mazzaferri
Sep 17, 1984·Biochemical and Biophysical Research Communications·B AmiranoffM Laburthe
Mar 15, 1984·Biochemical and Biophysical Research Communications·O MiyanagaG L Cottam
Aug 11, 1995·Molecular and Cellular Endocrinology·A C LowethN G Morgan
Jul 5, 1994·Biochemical Pharmacology·A B MukherjeeN Pattabiraman
Mar 1, 1993·Diabetes·J TurkS Ramanadham
Jul 1, 1996·Acta Physiologica Scandinavica·C H McIntoshR A Pederson
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·D E Clapham, E J Neer
May 9, 1997·The Journal of Biological Chemistry·A SauvadetC Pavoine
Jun 27, 1997·The Journal of Biological Chemistry·J Balsinde, E A Dennis
Jun 9, 1997·Biochemical and Biophysical Research Communications·A KubotaY Seino
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·H C LinA G Gilman
Nov 26, 1998·The Journal of Biological Chemistry·T J Shuttleworth, J L Thompson
Dec 29, 1998·The Journal of Biological Chemistry·B A RzigalinskiE F Ellis
Jan 15, 1999·Pharmacology & Therapeutics·J Wess

❮ Previous
Next ❯

Citations

Mar 28, 2012·Journal of Diabetes Investigation·Christopher Hs McIntoshSu-Jin Kim
Dec 17, 2008·Pharmacological Reviews·Wook Kim, Josephine M Egan
Dec 3, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chin Chen, Eric Sibley
Apr 4, 2014·International Journal of Molecular Sciences·Barbara MainoSebastiano Cavallaro
Jun 16, 2011·Archives of Physiology and Biochemistry·William D BradleyCristina M Rondinone
May 15, 2007·Gastroenterology·Laurie L Baggio, Daniel J Drucker
Jul 17, 2004·Regulatory Peptides·Li XieZheng-Wang Chen
Feb 18, 2004·Diabetologia·T Vilsbøll, J J Holst
Apr 13, 2007·Archives of Biochemistry and Biophysics·Victor A GaultPeter R Flatt
Jan 8, 2004·American Journal of Physiology. Endocrinology and Metabolism·Ke-Hong DingCarlos M Isales
Jul 24, 2004·American Journal of Physiology. Endocrinology and Metabolism·Jens Juul Holst, Jesper Gromada
Feb 12, 2019·Scientific Reports·Seul Ji LeeSung Won Kwon
Mar 29, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Wendell J LuPatrick Tso
Mar 5, 2003·Pharmacological Reviews·Kelly E MayoDaniel J Drucker
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Francis C LynnRaymond A Pederson
Jan 24, 2003·American Journal of Physiology. Endocrinology and Metabolism·Nathalie PamirRaymond A Pederson

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.