A new pathway for the synthesis of α-ribazole-phosphate in Listeria innocua.

Molecular Microbiology
Michael J Gray, Jorge C Escalante-Semerena

Abstract

The genomes of Listeria spp. encode all but one of 25 enzymes required for the biosynthesis of adenosylcobalamin (AdoCbl; coenzyme B(12) ). Notably, all Listeria genomes lack CobT, the nicotinamide mononucleotide:5,6-dimethylbenzimidazole (DMB) phosphoribosyltransferase (EC 2.4.2.21) enzyme that synthesizes the unique α-linked nucleotide N(1) -(5-phospho-α-D-ribosyl)-DMB (α-ribazole-5'-P, α-RP), a precursor of AdoCbl. We have uncovered a new pathway for the synthesis of α-RP in Listeria innocua that circumvents the lack of CobT. The cblT and cblS genes (locus tags lin1153 and lin1110) of L. innocua encode an α-ribazole (α-R) transporter and an α-R kinase respectively. Results from in vivo experiments indicate that L. innocua depends on CblT and CblS activities to salvage exogenous α-R, allowing conversion of the incomplete corrinoid cobinamide (Cbi) into AdoCbl. Expression of the L. innocua cblT and cblS genes restored AdoCbl synthesis from Cbi and α-R in a Salmonella enterica cobT strain. LinCblT transported α-R across the cell membrane, but not α-RP or DMB. UV-visible spectroscopy and mass spectrometry data identified α-RP as the product of the ATP-dependent α-R kinase activity of LinCblS. Bioinformatics analyses suggest that...Continue Reading

References

May 1, 1987·Journal of Bacteriology·J C Escalante-Semerena, J R Roth
Jul 1, 1968·Journal of Bacteriology·D BerkowitzB N Ames
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·L A Maggio-Hall, J C Escalante-Semerena
Jun 30, 2000·Protein Expression and Purification·R B Kapust, D S Waugh
Mar 22, 2003·FEMS Immunology and Medical Microbiology·Carmen BuchrieserUNKNOWN Listeria Consortium
Jul 19, 2003·The Journal of Biological Chemistry·Dmitry A RodionovMikhail S Gelfand
Jan 20, 2004·Journal of Bacteriology·Giuseppe Bertani
Dec 31, 2005·Nucleic Acids Research·Victor M MarkowitzNikos C Kyrpides
Feb 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael J Gray, Jorge C Escalante-Semerena
Mar 23, 2007·Nature·Michiko E TagaGraham C Walker
Mar 4, 2008·Biochemistry·Kathryn M McCullochSteven E Ealick
Oct 22, 2008·Journal of Bacteriology·Dmitry A RodionovThomas Eitinger

❮ Previous
Next ❯

Citations

Nov 29, 2014·Frontiers in Microbiology·Terence S CroftsMichiko E Taga
Jul 30, 2014·Frontiers in Microbiology·Erica C Seth, Michiko E Taga
Jul 1, 2016·Annals of the New York Academy of Sciences·Hans K Biesalski
Feb 18, 2016·The ISME Journal·Winifred M JohnsonElizabeth B Kujawinski
Jun 24, 2011·Molecular Microbiology·Chi Ho Chan, Jorge C Escalante-Semerena
Oct 28, 2017·Frontiers in Microbiology·Gerrit WienhausenMeinhard Simon
Jul 8, 2020·Science·Olga M SokolovskayaMichiko E Taga
Oct 16, 2018·Frontiers in Microbiology·Andrea Carolina TorresMaría Pía Taranto
Jun 11, 2020·FEMS Microbiology Ecology·Winifred M JohnsonHeidi Smith
Jun 10, 2021·Biochemistry·Theodoric A MattesJorge C Escalante-Semerena

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.