A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood

The Journal of Parasitology
Olof HellgrenStaffan Bensch

Abstract

Many bird species host several lineages of apicomplexan blood parasites (Protista spp., Haemosporida spp.), some of which are shared across different host species. To understand such complex systems, it is essential to consider the fact that different lineages, species, and families of parasites can occur in the same population, as well as in the same individual bird, and that these parasites may compete or interact with each other. In this study, we present a new polymerase chain reaction (PCR) protocol that, for the first time, enables simultaneous typing of species from the 3 most common avian blood parasite genera (Haemoproteus, Plasmodium, and Leucocytozoon). By combining the high detection rate of a nested PCR with another PCR step to separate species of Plasmodium and Haemoproteus from Leucocytozoon, this procedure provides an easy, rapid, and accurate method to separate and investigate these parasites within a blood sample. We have applied this method to bird species with known infections of Leucocytozoon spp., Plasmodium spp., and Haemoproteus spp. To obtain a higher number of parasite lineages and to test the repeatability of the method, we also applied it to blood samples from bluethroats (Luscinia svecica), for whic...Continue Reading

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Citations

Sep 27, 2008·Parasitology Research·Joaquín Ortego, Pedro J Cordero
Aug 16, 2011·Parasitology Research·Laryssa HoweMaurice R Alley
Aug 10, 2012·Parasitology Research·Shauna M BaillieDianne H Brunton
Dec 12, 2012·Parasitology Research·Petr SynekJan Votýpka
Feb 23, 2013·Parasitology Research·A C NorteJ A Ramos
Jun 15, 2011·Oecologia·Elizabeth YohannesStaffan Bensch
Nov 15, 2011·Parasitology·Staffan BenschJosé Luis Copete
Aug 21, 2013·Parasitology·Patricia SilveiraÉrika M Braga
Oct 7, 2008·New Zealand Veterinary Journal·M R AlleyT Atkinson
Oct 11, 2013·Vector Borne and Zoonotic Diseases·Joseph O Mehus, Jefferson A Vaughan
Nov 4, 2011·Proceedings. Biological Sciences·Janske van de CrommenackerJan Komdeur
Jan 31, 2013·Malaria Journal·Juan van RooyenPhilippe Christe
Feb 22, 2007·PloS One·Javier Pérez-TrisStaffan Bensch
Jun 19, 2008·PloS One·Martin StjernmanJan-Ake Nilsson
Dec 25, 2012·The Journal of Parasitology·Cynthia Martins VillarSilvia Nassif Del Lama
Jun 21, 2008·The Journal of Parasitology·Eszter SzöllsiDennis Hasselquist
Jul 9, 2008·The Journal of Parasitology·O KroneS Bensch
Jun 26, 2008·The Journal of Parasitology·Gediminas ValkiunasStaffan Bensch

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Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

BioEdit
MEGA

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