A new PCR-based seroneutralization assay in cell culture for diagnosis of hepatitis E.

Journal of Clinical Microbiology
J MengJ Pillot

Abstract

A new method for the serological diagnosis of hepatitis E virus (HEV) infection based on neutralization of the virus in cell culture was developed. The test involves a short incubation of the virus in the presence of the serum sample to be tested and permissive cells. With viral replication being limited and without a cytopathic effect, viral growth in cells is evaluated by reverse transcription and PCR. The specificity of the test was established by studying sera from healthy individuals and patients with hepatitis living in France, where autochthonous hepatitis E is unknown. The kinetics and sensitivity of antibody detection were evaluated during the experimental infection of monkeys. Neutralizing antibodies were found in 79% of patients during an outbreak of hepatitis E and in 43% of patients with sporadic, acute non-A, non-B (without anti-hepatitic C virus antibodies) hepatitis. This neutralization assay is proposed as a confirmatory test for the available enzyme-linked immunosorbent assay (ELISA), which is now recognized as giving many false-positive reactions, and to improve identification of new hepatitis viruses since false-negative reactions with HEV ELISA are also encountered.

References

Feb 1, 1995·The Journal of Infectious Diseases·V A ArankalleR H Purcell
Sep 10, 1994·Lancet·D LavanchyP C Frei
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·S A TsarevR H Purcell
Jun 1, 1994·Journal of Medical Virology·S A TsarevR H Purcell
Apr 1, 1993·Gastroenterology·R H Purcell
Mar 2, 1996·Lancet·A J Zuckerman

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Citations

Jul 9, 2004·World Journal of Gastroenterology : WJG·Guo-Bing Chen, Ji-Hong Meng
Dec 20, 2007·The Journal of General Virology·Shuizhen HeNingshao Xia
Jul 11, 2017·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Rosina Girones
Jul 6, 2004·Drugs·Harald Claus Worm, Gerhard Wirnsberger
Feb 12, 2009·Journal of Biosciences·Vivek ChandraShahid Jameel
Jul 22, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tetsuo YamashitaYoshiharu Matsuura
Sep 21, 2010·Uirusu·Hiroaki Okamoto
Aug 24, 2013·International Journal of STD & AIDS·Amitis RamezaniArezoo Aghakhani
Mar 12, 2010·The Journal of General Virology·Zi-Zheng ZhengNing-Shao Xia
Mar 9, 2017·Human Vaccines & Immunotherapeutics·Xing WuZhenglun Liang
May 5, 2017·Food and Environmental Virology·Nigel CookReimar Johne
Oct 8, 2018·Acta Tropica·Luan Araújo BezerraMaria Rosângela Cunha Duarte Coêlho
May 1, 2018·Human Vaccines & Immunotherapeutics·Yufeng CaoXinping Wang
Jul 20, 2004·World Journal of Gastroenterology : WJG·Ling Wang, Hui Zhuang
Feb 28, 2007·The Journal of General Virology·Toshinori TanakaHiroaki Okamoto
Jul 7, 2019·Viruses·Rebecca Menhua FuViet Loan Dao Thi
Mar 14, 2018·Cold Spring Harbor Perspectives in Medicine·Bruce L Innis, Julia A Lynch
Sep 3, 1999·Clinical and Diagnostic Laboratory Immunology·R HuangM Liu

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