PMID: 2123611Oct 1, 1990Paper

A new perspective of microbial survival and dissemination in a prospectively contaminated air-fluidized bed model

American Journal of Infection Control
W D Winters

Abstract

A major concern of users of air-fluidized beds has been the possibility that such beds might be a source of microbial contamination. The purpose of this series of prospective, controlled experiments was to measure quantitatively the dissemination and survival of Bacillus subtilis, a species of bacterium that forms desiccation-resistant spores, as it was associated with circulating and clumped microbeads after challenge in an air-fluidized bed operating under decontamination conditions of heating at 48 degrees C and microbead agitation by an air flow of 100% at 110 cu ft/min. Microbead samples collected after B. subtilis challenge from predesignated depths and locations within the air-fluidized bed at 0.25, 1, 2, 4, 24, and 48 hours were assayed for colony-forming units (CFU) of challenge bacteria by end point dilution and streak-plate assays. Results of three experiments with an average challenge of 1110 CFU of B. subtilis per gram of microbeads indicated that no more than 0.46 CFU of B. subtilis per gram of circulating microbeads and 0.78 CFU per gram of clumped microbeads were detected after 24 to 48 hours at decontamination conditions. Few microbes were detected during three control (sterile water) challenges of the air-flui...Continue Reading

References

Feb 1, 1986·American Journal of Infection Control·D VesleyJ L Lauer
May 1, 1973·The American Surgeon·R J SharbaughF A Wright
Mar 1, 1983·The Journal of Trauma·A Scheidt, L M Drusin

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Citations

Mar 1, 1995·Intensive Care Medicine·D W Ryan
Sep 11, 1993·Lancet·M J Clancy
Dec 1, 1994·The Journal of Hospital Infection·M J Clancy

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