A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I

Journal of Cell Science
Edoardo PetriniPatrick Varga-Weisz

Abstract

Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a quiescence response that is dependent on the endocytic function of Myo1. Transcriptomic analysis revealed a wide perturbation of gene expression with induction of stress-regulated genes upon phosphate starvation in wild-type but not Δmyo1 cells. Thus, endocytosis plays a pivotal role in mediating the cellular response to nutrients, bridging the external environment and internal molecular functions of the cell.

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Citations

Apr 26, 2020·Nature Communications·Rostyslav MakarenkoBenoît Arcangioli
Dec 23, 2020·Yeast·Siyu Sun, David Gresham

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Datasets Mentioned

BETA
GSE67126

Methods Mentioned

BETA
ChIP-Seq
RNA-Seq

Software Mentioned

Imaris
BinGO
DESeq2
TopHat
SeqMonk
Cell F
NIS Elements
FIJI
ImageJ

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