A new pineoblastoma cell line, PER-480, with der(10)t(10;17), der(16)t(1;16), and enhanced MYC expression in the absence of gene amplification

Cancer Genetics and Cytogenetics
U R KeesJaclyn A Biegel

Abstract

Pineoblastoma is a rare, but highly malignant tumor of the central nervous system (CNS) in children and is classified as a central primitive neuroectodermal tumor (PNET). Despite notable recent advances in understanding the molecular genetic basis of malignancies, the pathogenesis of PNETs remains enigmatic. There is scant information on the cytogenetics of PNETs arising in the pineal gland and the only three reported cases did not show any common aberrations. Here we report the establishment and characterization of a new pineoblastoma cell line, PER-480. The biopsy material and the cell line were characterized using light and electron microscopy and immunohistochemical analyses. The cell line was examined for expression of cell surface markers using a panel of monoclonal antibodies and by cytogenetic analysis. MYC family genes were studied at the DNA, RNA, and protein level. Cell line PER-480 showed neuronal differentiation and the karyotype demonstrated two abnormalities, a der(10)t(10;17) and a der(16)t(1;16). An intriguing finding is that all three pineoblastoma cell lines established in our laboratory, PER-452, PER-453, and PER-480, showed enhanced expression but not amplification of a member of the MYC family of proto-onc...Continue Reading

References

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Citations

Jan 1, 2005·Acta Neuropathologica·Jacques ChampierMichelle Fèvre-Montange
May 19, 2005·Cancer Genetics and Cytogenetics·Peter B DallasUrsula R Kees
Mar 28, 2003·Cancer Genetics and Cytogenetics·Jeffrey R SawyerMark E Linskey
Dec 17, 2008·Neuropathology : Official Journal of the Japanese Society of Neuropathology·David J HolthouseUrsula R Kees
May 15, 2010·Future Oncology·Michelle Fèvre-MontangeAnne Jouvet
Jul 16, 2014·Acta Neuropathologica·Leanne de KockWilliam D Foulkes
Oct 3, 2006·Cancer Genetics and Cytogenetics·Angela E BrownDavid R Betts
Jul 1, 2014·Neuro-Chirurgie·A JouvetM Fèvre Montange
Dec 7, 2000·Genes, Chromosomes & Cancer·C H RickertW Paulus
Jan 28, 2005·Journal of Neuro-oncology·Torsten PietschJames T Rutka

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