PMID: 8582974Jul 1, 1995Paper

A new predictive test for in-vitro fertilization based on the induction of sperm acrosome reaction by N-acetylglucosamine-neoglycoprotein

Human Reproduction
A BrandelliJ G Tezon

Abstract

Neoglycoproteins with N-acetylglucosamine residues (BSA-GlcNAc) induced specifically the acrosome reaction (AR) in human spermatozoa. Our objective was to investigate the relationship between this phenomenon and the invitro fertilization (IVF) rate. Sperm suspensions from IVF protocols were incubated with BSA-GlcNAc (t), using calcium ionophore (i) or medium alone (c) as positive or negative controls. When the normalized AR percentage ratio (STIM) (% ARt-%ARc):(%ARi-%ARc) was compared with fertilization rate in 31 couples from our IVF programme, a positive correlation was found (r = 0.46, P < 0.01). The fertilization rate in patients with STIM > or = 0.2 was higher than in non-responders (STIM < 0.2); 72 +/- 7% compared with 5 +/- 3%. The overall predictive value of this test for adequate fertilization rate (> 30%) was 87%, sensitivity 91% and specificity 78%. False positives were 9% and false negatives 22%. For successful fertilization rates (> 60%), the results were: overall predictive value, 84%; sensitivity 100%; specificity 64%. False positives were 23% and no false negatives were found. The results indicated that the induction of AR in human spermatozoa by GlcNAc-neoglycoproteins could be used to predict their fertilizing...Continue Reading

Citations

Dec 22, 1998·Biology of Reproduction·C R Loeser, D R Tulsiani
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E P PassosA Brandelli
Jul 6, 2004·Molecular Reproduction and Development·S TangheA de Kruif
Jan 14, 2020·Pakistan Journal of Biological Sciences : PJBS·Salem Abd El-Hadi HabibMarwa Ebrahim Elafify
Jul 11, 2006·Molecular Human Reproduction·Karina ZittaPatricia V Miranda
Dec 29, 2000·Andrologia·T ZeginiadouS Mantalenakis
Jun 18, 1998·Clinical Biochemistry·A Brandelli, E P Passos

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