A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.

Applied and Environmental Microbiology
Martin J LoessnerSiegfried Scherer

Abstract

A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and differentiation of Listeria strains when the release of native nucleic acids or proteins is required.

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Citations

Feb 18, 2011·Bioengineered Bugs·Mark FentonAidan Coffey
Jan 7, 2003·Applied and Environmental Microbiology·Lisa GorskiRobert E Mandrell
Oct 4, 2012·Future Microbiology·Mathias SchmelcherMartin J Loessner
Jan 22, 2015·Critical Reviews in Biotechnology·Lorena Rodríguez-RubioPilar García
Nov 2, 2014·Frontiers in Microbiology·Hang YangHongping Wei
May 7, 2009·FEMS Microbiology Reviews·Sarah O'FlahertyAidan Coffey
Feb 8, 2006·Applied and Environmental Microbiology·Allan L DelisleMing Guo
Oct 17, 2006·Journal of Bacteriology·Maja BaumgärtnerLothar Jänsch
Jan 1, 2008·International Journal of Food Microbiology·Dongyou Liu

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