PMID: 7540820DOI: 10.1128/aem.61.3.1150-1152.1995Mar 1, 1995Paper
A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.
Applied and Environmental Microbiology
Martin J LoessnerSiegfried Scherer
Abstract
A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and differentiation of Listeria strains when the release of native nucleic acids or proteins is required.
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Citations
Jun 1, 1995·Molecular Microbiology·M J LoessnerS Scherer
Oct 31, 2002·Applied and Environmental Microbiology·Markus ZimmerMartin J Loessner
Sep 26, 2003·Molecular Microbiology·Markus ZimmerMartin J Loessner
Feb 18, 2011·Bioengineered Bugs·Mark FentonAidan Coffey
Mar 30, 2017·Antimicrobial Agents and Chemotherapy·Soo Youn JunSang Hyeon Kang
Jan 7, 2003·Applied and Environmental Microbiology·Lisa GorskiRobert E Mandrell
Jul 8, 2005·Microbiology·Marian TakácUdo Bläsi
May 4, 2011·Microbial Biotechnology·Mathias SchmelcherMartin J Loessner
Jan 19, 2013·Current Microbiology·Guangtao HuangFuquan Hu
Oct 4, 2012·Future Microbiology·Mathias SchmelcherMartin J Loessner
Jul 2, 2011·Applied Microbiology and Biotechnology·Mathias SchmelcherMartin J Loessner
Jan 22, 2015·Critical Reviews in Biotechnology·Lorena Rodríguez-RubioPilar García
Nov 2, 2014·Frontiers in Microbiology·Hang YangHongping Wei
Jan 6, 2009·Journal of Applied Microbiology·S WangX Zhang
May 7, 2009·FEMS Microbiology Reviews·Sarah O'FlahertyAidan Coffey
Feb 6, 2007·Applied and Environmental Microbiology·Jan W KretzerMartin J Loessner
Jan 23, 2014·Antimicrobial Agents and Chemotherapy·Soo Youn JunSang Hyeon Kang
Jan 29, 2000·Molecular Microbiology·M J LoessnerR Calendar
Feb 8, 2006·Applied and Environmental Microbiology·Allan L DelisleMing Guo
Apr 26, 2002·Molecular Microbiology·Martin J LoessnerSiegfried Scherer
Apr 21, 2017·Current Microbiology·Xue MengHongbing Shao
Jan 10, 2018·Scientific Reports·Sangjin ParkSang Hyeon Kang
May 1, 1997·Journal of Bacteriology·M J LoessnerS Scherer
Apr 7, 2009·Journal of Bacteriology·Heather S O'NeilHélène Marquis
Oct 29, 1997·Journal of Virology·E R JensenJ F Miller
Nov 1, 1995·Journal of Bacteriology·M J Loessner, S Scherer
Apr 1, 1996·Applied and Environmental Microbiology·M J LoessnerS Scherer
Aug 1, 1996·Applied and Environmental Microbiology·M J LoessnerS Scherer
Jun 25, 2005·Virology·Ta-Chun LoThy-Hou Lin
Oct 17, 2006·Journal of Bacteriology·Maja BaumgärtnerLothar Jänsch
Jan 1, 2008·International Journal of Food Microbiology·Dongyou Liu
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