PMID: 9537040Jan 1, 1980Paper

A new quantitative fluorimetric assay for phagocytosis of bacteria

Scandinavian Journal of Immunology
B VrayA D Strosberg

Abstract

We describe a new quantitative fluorimetric assay for phagocytosis of bacteria. A suspension of fluorescein-labelled bacteria (Micrococcus lysodeikticus) is mixed and incubated with phagocytes. After fixation with paraformaldehyde, the excess non-phagocytosed and adsorbed bacteria are lysed with a solution of lysozyme in phosphate-buffered saline. After washing of the phagocytes, the fluorescence of those that have ingested the labelled bacteria is measured with a spectrofluorimeter. We report results obtained with different types of phagocytes which show that this method allows sensitivity, saturation and kinetic studies and the calculation of a phagocytic index.

References

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Citations

Jan 12, 1999·Biulleten' eksperimental'noĭ biologii i meditsiny·N V Masnaia, G M Ratner
Sep 24, 2008·Clinical Oral Investigations·Christian HannigMatthias Hannig
Dec 8, 2009·Clinical Oral Investigations·Christian HannigMatthias Hannig
Nov 12, 2005·Bulletin of Experimental Biology and Medicine·E D Gol'dbergA A Churin
Aug 9, 1988·Journal of Immunological Methods·J A Oben, J C Foreman
Sep 14, 1994·Journal of Immunological Methods·N Plasman, B Vray
Nov 1, 1988·Immunopharmacology·J A Oben, J C Foreman
Sep 1, 2015·European Journal of Oral Sciences·Susann GrychtolChristian Hannig
Mar 11, 2015·Clinical Oral Investigations·Elżbieta PaszynskaChristian Hannig
Sep 2, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Carolin BoehlkeOliver Zierau
Sep 1, 1986·Biochemical Pharmacology·F AntoniI Csuka
Jul 9, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Carolin BoehlkeChristian Hannig

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