Mar 31, 2005

A new recombinant cell-based bioluminescent assay for sensitive androgen-like compound detection

Biosensors & Bioelectronics
Elisa MicheliniAldo Roda

Abstract

A public concern is continuously arising about the presence of natural and anthropogenic compounds which affect human health by modulating normal endocrine functions. These substances, defined as endocrine disrupting compounds (EDC) represent an heterogeneous class of molecules either steroidal or not, sharing the ability of interfering with the endocrine system via nuclear receptor signaling pathways. Therefore there is an urgent need for high throughput screening systems able to detect EDCs and evaluate their biological activity. However, little attention has been dedicated to the development of assays for androgen-like compounds. The present work describes the development and optimization of a new rapid and sensitive bioluminescent yeast-based bioassay for androgen-like compounds in a 96-well microplate format. The bioassay is based on recombinant Saccharomyces cerevisiae cells modified to express human androgen receptor (hAR) and containing the sequence androgen response element (ARE) which drives the expression of Photinus pyralis luciferase, used as reporter gene. A recombinant yeast strain constitutively expressing luciferase was used as external control to correct the light signal accordingly to cell viability and sampl...Continue Reading

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Mentioned in this Paper

Pathologic Cytolysis
Androgen Receptor
Biochemical Pathway
Testosterone Measurement
Ethanol
Androgen Effect
Cross-talk
Ethanol Measurement
Histidine
Saccharomyces cerevisiae allergenic extract

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