A new recombinant mosquito salivary allergen, rAed a 2: allergenicity, clinical relevance, and cross-reactivity

Allergy
Z PengF E Simons

Abstract

Mosquito salivary proteins cause allergic reactions in humans. The allergenicity, clinical relevance, and species cross-reactivity of a new 37-kDa recombinant mosquito (Aedes aegypti) salivary allergen, rAed a 2, were evaluated. rAed a 2 was expressed using a baculovirus/insect cell system and purified. Its allergenicity was examined using an enzyme-linked immunosorbent assay (ELISA), ELISA inhibition tests, immunoblots, and skin tests. Epicutaneous tests with the allergen, mosquito whole body extracts, and mosquito bite tests were performed on 48 volunteers. Serum rAed a 2-specific immunoglobulin E (IgE) was measured in individuals with positive mosquito saliva-specific IgE and negative controls. Both immunoblots and ELISAs demonstrated that rAed a 2 bound to the IgE of mosquito-allergic individuals. The binding could be inhibited by the addition of a natural mosquito preparation. Furthermore, rAed a 2 induced immediate and delayed skin reactions. Ten per cent of 31 participants with a positive mosquito bite test had positive skin reactions to rAed a 2, compared with 32% for mosquito whole body extract. None of the participants with a negative bite test showed positive reactions to either of the two extracts. Forty-three per c...Continue Reading

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Citations

Aug 28, 2007·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Qiang WangZhikang Peng
Nov 15, 2012·BMC Genomics·Albin FontaineLionel Almeras
Sep 29, 2011·Parasites & Vectors·Albin FontaineLionel Almeras
May 30, 2014·The Journal of Infectious Diseases·Christopher N MoresSilas A Davidson
Apr 8, 2014·Mediators of Inflammation·Ji-Fu WeiShaoheng He
Jan 29, 2013·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Howard C Crisp, Kimberly S Johnson
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May 21, 2015·Cancer Science·Yasuhisa SakakibaraAkihiro Yachie
Jul 8, 2016·The World Allergy Organization Journal·Luis CaraballoVictoria Cardona
Jul 11, 2007·Current Opinion in Allergy and Clinical Immunology·Zhikang Peng, F Estelle R Simons
Oct 3, 2018·Tropical Medicine and Infectious Disease·André B SagnaFranck Remoue
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Mar 6, 2019·BMC Genomics·Vera Margarete ScarpassaJosé M C Ribeiro
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Feb 27, 2020·The World Allergy Organization Journal·Anunya OpasawatchaiPonpan Matangkasombut
May 10, 2007·Current Opinion in Allergy and Clinical Immunology
Jul 11, 2007·Current Opinion in Allergy and Clinical Immunology

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