PMID: 8948506Nov 1, 1996Paper

A new reliable chemiluminescence immunoassay (CLIA) for prostaglandin E2 using enhanced luminol as substrate

Prostaglandins
W NeupertG Geisslinger

Abstract

A sensitive and reliable chemiluminescence immunoassay suitable for the quantitative determination of prostaglandin E2 (PGE2) has been developed using 96 well microtiter plates (MTP). The assay is based on a competitive reaction between a highly specific monoclonal anti-PGE2 antibody (mouse), free antigen and solid phase bound antigen. The MTP was first coated with a bovine serum albumin (BSA)-PGE2 conjugate. Then, after preincubating, the anti-PGE2 antibody (Ab) and the analyte were added. The remaining amount of free antibody was captured by the solid phase bound BSA-PGE2 conjugate. The monoclonal antibody captured on the MTP was determined using biotinylated anti-mouse-Ab and a complex of avidin and biotin-labelled horseradish peroxidase (HRP). Substrate for HRP was the cyclic diacyl hydrazide compound luminol, enhanced by p-iodophenol. Photons emitted during the reaction were measured using a photomultiplier tube. The assay has been validated with assay buffer and human plasma over a concentration range of 10-50,000 pg/ml. The lower limit of quantification is 100 pg/ml (2 pg/well) and 150 pg/ml (3 pg/well) for buffer and plasma, respectively. The intra-day coefficients of variation (CV) for the range of 100-50,000 pg/ml are...Continue Reading

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Citations

Oct 31, 2008·Talanta·C DodeigneR Lejeune
Oct 24, 1998·Prostaglandins & Other Lipid Mediators·H JohnW Schlegel
Nov 8, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·B M Zamora, M Hartung
Apr 23, 2014·Applied Biochemistry and Biotechnology·Parvez KhanMd Imtaiyaz Hassan
Jul 21, 2012·Analytical and Bioanalytical Chemistry·Katrin StrassburgRob J Vreeken
Mar 18, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Chun WuYoshihiro Ohmiya
Jan 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ivan LiakhAdriana Mika
Feb 5, 2019·Journal of Chemical Theory and Computation·Ling Yue, Ya-Jun Liu

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