A new role for E12/E47 in the repression of E-cadherin expression and epithelial-mesenchymal transitions

The Journal of Biological Chemistry
Mirna A Pérez-MorenoAmparo Cano

Abstract

Down-regulation of E-cadherin expression is a determinant of tumor cell invasiveness, an event frequently associated with epithelial-mesenchymal transitions. Here we show that the mouse E12/E47 basic helix-loop-helix transcription factor (the E2A gene product) acts as a repressor of E-cadherin expression and triggers epithelial-mesenchymal transitions. The mouse E47 factor was isolated in a one-hybrid system designed to isolate repressors of the mouse E-cadherin promoter. Epithelial cells ectopically expressing E47 adopt a fibroblastic phenotype and acquire tumorigenic and migratory/invasive properties, concomitant with the suppression of E-cadherin expression. Suppression of E-cadherin expression under stable or inducible expression of E47 in epithelial cells occurs at the transcriptional level and is dependent on the E-boxes of the E-cadherin promoter. Interestingly, analysis of endogenous E2A expression in murine and human cell lines illustrated its presence in E-cadherin-deficient, invasive carcinoma cells but its absence from epithelial cell lines. This expression pattern is consistent with that observed in early mouse embryos, where E2A mRNA is absent from epithelia but strongly expressed in the mesoderm. These results im...Continue Reading

References

Jun 1, 1992·Seminars in Cell Biology·J BehrensW Birchmeier
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J BehrensW Birchmeier
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·K YoshiuraS Hirohashi
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A BlanarW J Rutter
Aug 1, 1995·Molecular and Cellular Biology·C P Shen, T Kadesch
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·L LarueR Kemler
May 1, 1994·Nature Genetics·J I RisingerJ Boyd
Oct 1, 1993·Current Opinion in Cell Biology·M Takeichi
Jan 1, 1993·Annual Review of Cell Biology·W G Stetler-StevensonL A Liotta
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·V J RobertsC Murre
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A AronheimM D Walker
Jan 5, 1996·The Journal of Biological Chemistry·G HennigJ Behrens
Oct 1, 1995·Current Opinion in Cell Biology·M Takeichi
Oct 1, 1996·Current Opinion in Cell Biology·O HuberR Kemler
Feb 1, 1997·Current Opinion in Neurobiology·J E Lee
Jun 20, 1998·Molecular and Cellular Biology·Y ZhuangM Dai
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·I Engel, C Murre
Oct 6, 1999·Current Opinion in Genetics & Development·I Engel, C Murre
Dec 28, 1999·Molecular and Cellular Biology·M E Massari, C Murre
Apr 6, 2000·Journal of the National Cancer Institute·G TamuraS J Meltzer

❮ Previous
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Citations

May 15, 2002·Genes, Chromosomes & Cancer·Karen M Hajra, Eric R Fearon
Dec 6, 2005·Virchows Archiv : an International Journal of Pathology·Erika RosivatzMario Sarbia
Nov 6, 2007·Clinical & Experimental Metastasis·Geert BerxJonathan P Sleeman
Feb 1, 2008·Clinical & Experimental Metastasis·Sharon BarrJohn D Haley
Jun 15, 2007·Journal of Mammary Gland Biology and Neoplasia·Georgia AgiostratidouRachel B Hazan
Nov 19, 2009·Journal of Biosciences·Maithili P DalviAnandwardhan A Hardikar
Dec 16, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·H Peinado, A Cano
Dec 31, 2003·Gene·Kikumi Hata, Junichiro Mizuguchi
Nov 5, 2002·The American Journal of Pathology·Erika RosivatzKarl-Friedrich Becker
Apr 12, 2003·Experimental Cell Research·Jean-Philippe CoppéPierre-Yves Desprez
Jan 21, 2009·Cell Research·Jian XuRik Derynck
Feb 9, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Robert StraussJiri Bartek
May 6, 2008·Nature Cell Biology·Eric A Miska
Oct 17, 2009·Nature Protocols·Gema Moreno-BuenoAmparo Cano
Feb 18, 2004·Nature Reviews. Cancer·Ugo Cavallaro, Gerhard Christofori
May 18, 2007·Nature Reviews. Cancer·Héctor PeinadoAmparo Cano
Jan 25, 2013·Nature Reviews. Cancer·Bram De Craene, Geert Berx
Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Aug 24, 2005·Oncogene·Yan-Nan LiuJi Hshiung Chen
Mar 29, 2006·Oncogene·C FrancíA García de Herreros
Jul 18, 2008·Molecular BioSystems·Marc P Stemmler
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Irene E WhitneyBenjamin E Reese
Feb 15, 2011·The Journal of Biological Chemistry·Natàlia DaveAntonio García de Herreros
Mar 24, 2009·Nucleic Acids Research·C Benjamin LaiDixie L Mager
Aug 19, 2011·Nucleic Acids Research·Jesús EspadaManel Esteller
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Wenxiang Meng, Masatoshi Takeichi
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Julian Heuberger, Walter Birchmeier
Sep 17, 2008·Molecular and Cellular Biology·Yan-Nian LiuJi-Hshiung Chen
Apr 10, 2002·Molecular and Cellular Biology·Chuan-ju LiuPeter Lengyel

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