A new selection method for pepper transformation: callus-mediated shoot formation

Plant Cell Reports
Y H LeeC H Harn

Abstract

We used two genes, TMV-CP and PPI1 (pepper-PMMV interaction 1 transcription factor), to transform commercially important chili pepper (Capsicum annuum) inbred lines (P915, P409) by means of Agrobacterium co-culture. Eighteen independently transformed T0 plants were obtained. The most critical point in the pepper transformation protocol was the selection of shoots growing on calli--referred to as callus-mediated shoot formation (indirect shooting)--because shoots not grown from the callus (direct shooting from the wounded surface) developed into non-transformants. Selection of the correct right callus type also proved to be an important requirement for obtaining transformed peppers. Six different types of callus developed during the selection process. Shoots regenerated from two of these types, while one type regenerated significantly more shoots than the other types, suggesting that the capacity for shoot formation is callus type-specific. Although the transformation rate was low, transformation via callus-mediated shoot formation proved to be reproducible and was confirmed by Southern and Northern blot analyses. Based on the experimental data, we have succeeded in developing a new protocol for the selection and transformation ...Continue Reading

Citations

Nov 20, 2008·Plant Cell Reports·Yun Hee LeeChee Hark Harn
Dec 17, 2010·Plant Cell Reports·Cesar Aza-GonzálezNeftalí Ochoa-Alejo
Feb 10, 2011·Plant Cell Reports·Iris HeidmannKim Boutilier
Sep 12, 2009·Biotechnology Advances·S L KothariN Ochoa-Alejo
Oct 2, 2013·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·R Vinoth KumarS Chakraborty
Apr 16, 2019·Plant Signaling & Behavior·Hyeran Kim, Jongseok Lim
Oct 14, 2018·International Journal of Molecular Sciences·Raihana RidzuanMagaji Usman
May 1, 2021·International Journal of Molecular Sciences·Sung-Il ParkHyeran Kim

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