A new sensitive and automated chemiluminescent microparticle immunoassay for quantitative determination of hepatitis C virus core antigen

Journal of Virological Methods
Kaori MorotaKatsumichi Takeda

Abstract

A new sensitive and automated chemiluminescent assay was developed for the quantitative determination of hepatitis C virus (HCV) core antigen (Ag) in human sera or plasma: the Abbott ARCHITECT HCV Ag test. The assay sensitivity was determined by testing 10 commercial HCV seroconversion panels. Without exception, a positive result for HCV core Ag was observed before anti-HCV detection, resulting in an average reduction in the period between exposure and detection of 35.8 days. Both HCV core Ag and HCV RNA were detected in the panels at the same time, indicating equivalent sensitivity and detectability. A total of 197 HCV specimens comprising genotypes 1a, 1b, 2a, 2b, 3a, 3k, 4a, 5a and 6a were evaluated. Among these, 196 (99.5%), 191 (97%) and 193 (98%) were reactive using the HCV Ag, the immunoradiometric HCV Ag and the Amplicor HCV Monitor 2 assays, respectively. A comparison with the Amplicor HCV Monitor 2 showed a correlation coefficient (r) of 0.74. The specificity of the assay was established at 99.8% by testing 5403 specimens from US volunteer blood donors, hospitalized patients and individuals with medical conditions unrelated to HCV infection, in addition to specimens containing potentially interfering substances.

References

Nov 26, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C NiederauD Häussinger
Jan 6, 2000·Journal of Hepatology·J M Pawlotsky
Aug 1, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E TanakaH J Alter
Jun 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Magali Bouvier-AliasJean-Michel Pawlotsky
Oct 18, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P DalyJ Buxton
Nov 28, 2002·Journal of Clinical Microbiology·Sandra S Richter
Mar 12, 2003·Journal of Medical Virology·Alessandro R ZanettiCharles Tackney
Sep 3, 2003·Journal of Virological Methods·Ryoichi SaitoNoboru Okamura
Oct 16, 2004·The Journal of General Virology·Peter Simmonds
Jun 7, 2005·Transfusion·Simone A GlynnMichael P Busch
Sep 9, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Peter SimmondsAnders Widell

❮ Previous
Next ❯

Citations

Nov 25, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A AlsiöUNKNOWN NORDynamIC Study Group
Jan 29, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ingmar MederackeHans L Tillmann
Oct 20, 2012·Clinical and Vaccine Immunology : CVI·Nozomi SakamakiHarufumi Tsuge
Mar 31, 2012·Journal of Clinical Microbiology·Yuan-Hung KuoSheng-Nan Lu
Dec 15, 2010·Journal of Virology·Anna AlbeckaJean Dubuisson
Aug 12, 2010·Indian Journal of Pathology & Microbiology·Natasha AliMohammad Khurshid
Apr 8, 2014·World Journal of Gastroenterology : WJG·Verónica SaludesElisa Martró
Mar 29, 2014·Asian Journal of Transfusion Science·Ekta GuptaAashish Choudhary
Mar 19, 2014·Arthritis Research & Therapy·Sabino RussiFranco Dammacco
Apr 25, 2014·BMC Infectious Diseases·Anna Rosa GarbugliaPatrizia Bagnarelli
Apr 14, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Tonya Mixson-HaydenSaleem Kamili
Feb 28, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Maria Cristina MediciAdriana Calderaro
Dec 19, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Elisabetta LoggiPietro Andreone
Nov 5, 2011·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Pelin YukselBekir Kocazeybek
May 31, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Maria Cristina MediciCarlo Chezzi
Mar 8, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Tapio SeiskariJanne Aittoniemi
Mar 18, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Marcel MiedougeJacques Izopet
Sep 22, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ingmar MederackeHans Ludger Tillmann
Mar 8, 2016·American Journal of Infection Control·Cuong Minh Duong, Mary-Louise McLaws
Jul 31, 2013·Journal of Medical Virology·Emanuele Durante-MangoniGiuseppe Portella
Jun 24, 2015·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Bo FengLai Wei
Nov 21, 2013·Journal of Viral Hepatitis·B HeidrichH Wedemeyer
Dec 9, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nikolien van de VenAndrew Hill
Jul 20, 2011·Diagnostic Microbiology and Infectious Disease·Koray ErgünayGülşen Hasçelik
Dec 20, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ingmar MederackeHans Ludger Tillmann
Jun 16, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Johannes VermehrenChristoph Sarrazin
Dec 16, 2010·The Korean journal of laboratory medicine·Dual SongHan Chul Son
Dec 19, 2014·American Journal of Public Health·Melissa G CollierRichard S Garfein
Jun 9, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ke-Qin Hu, Wei Cui
Oct 22, 2016·Luminescence : the Journal of Biological and Chemical Luminescence·Manwen YaoChengzhi Fang
Sep 11, 2016·Annals of Clinical Biochemistry·Lijuan WangGuojun Zhang
Apr 28, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Claudio GalliMario Plebani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Ingmar MederackeH L Tillmann
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Marcel MiedougeJacques Izopet
© 2021 Meta ULC. All rights reserved