PMID: 6990233Apr 1, 1980Paper

A new, sensitive and simple bioluminescence test for mutagenic compounds

Mutation Research
S UlitzurS Yannai

Abstract

A spontaneous dark variant of the luminous bacterium Photobacterium leiognathi was isolated. The reversion frequency of this variant to genetic-hereditary luminescent cells is greatly increased by nanogram quantities of different base-substitution and frameshift agents. This makes it possible to detect mutagenic compounds at concentrations 100 times lower than that detected by the Ames Test. Curing agents, such as acridine dyes, ethidium bromide and sodium dodecyl sulfate, are also very active in the reversion of this dark variant to the luminous state, but fail to revert it to a genetic-hereditary luminescent type. The nature of the primary mutation in the dark variant, and the potential use of this luminescence system for detecting different classes of carcinogenic chemical, are discussed.

References

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Citations

Mar 28, 2003·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jianli Guo, Yaliang Sun
Jan 12, 2005·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yaliang SunYiyong Li
Jan 4, 2006·Journal of Materials Science. Materials in Medicine·Y NomuraK Arizono
Jul 1, 1983·Mutation Research·M BarakD Merzbach
Jan 1, 1994·Research in Microbiology·D van der LelieM Mergeay
Jan 1, 1992·Biosensors & Bioelectronics·S LeeI Karube
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Ulitzur, I Weiser
Aug 31, 2010·Applied Microbiology and Biotechnology·Johan RobbensRonny Blust
Sep 27, 2005·Mutation Research·Claudia ZaniSilvano Monarca
May 19, 2006·Letters in Applied Microbiology·B Podgórska, G Wegrzyn
Jan 1, 1990·Environmental Pollution·K K KwanD Liu
Jun 1, 1984·The Journal of Applied Bacteriology·A NavehS Ulitzur
May 22, 2007·Ecotoxicology and Environmental Safety·Beata PodgórskaGrzegorz Wegrzyn
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·S Ulitzur
Apr 1, 1988·Journal of Bioluminescence and Chemiluminescence·S Ulitzur, M Barak
Jul 1, 1989·Journal of Bioluminescence and Chemiluminescence·A BerkovichS Ulitzur
Jul 15, 1985·International Journal of Cancer. Journal International Du Cancer·R HeilbronnH Zur Hausen
Feb 1, 1986·Bulletin of Environmental Contamination and Toxicology·I E YatesJ L Lanier
Sep 1, 1982·Mutation Research·T Gichner, J Velemínský
Feb 1, 1990·Journal of Bacteriology·D W Schultz, M Yarus

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