A new sensitive approach for spectrofluorimetric assay of Milnacipran and Amisulpride in real plasma and pharmaceutical preparations via complexation with Eosin Y dye

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Noha N AtiaWalid E Eltoukhi

Abstract

An accurate, economic, rapid, reliable spectrofluorimetric assay was developing for the assay of definite anti-depressant drugs namely Amisulpride and Milnacipran hydrochloride, in those pharmaceutical preparation and biological fluid. The suggested method was established on the detection of quenching process resulting from the action of AMS and MCN to the native fluorescent EosinY. A binary complex between selected medications and Eosin Y was established in acetate buffer (0.2 M) at pH 3.3 & 4.0 for AMS and MCN respectively. The relative fluorescence capacity was determined at λex = 301.8 nm and λem = 542.7 nm. The calibration graphs had been linear through extent from 0.02 to 0.3 and 0.1-1 μg mL-1, to both dugs respectively. Detection limits have been 0.0047 & 0.0188 μg mL-1 while quantitation limits have been 0.0141 & 0.0569 μg mL-1 to AMS & MCN respectively. Developed assay has been validated in agreement with ICH recommendations. Due to high sensitivity of the described assay, it allows the quantitation of anti-depressant drugs through biological fluid.

Citations

Jul 25, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Islam M MostafaSayed M Derayea
Jun 22, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Yongde LiuShuhu Xiao
Nov 4, 2020·Luminescence : the Journal of Biological and Chemical Luminescence·Muhammad N KhanMuhammad Mursaleen

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