A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus

Gene
A KissP Venetianer

Abstract

A new restriction endonuclease has been isolated from Bacillus sphaericus R. The purification procedure includes Bio-Gel filtration, (NH4)2SO4 fractionation and phosphocellulose chromatography. After the phosphocellulose step the enzyme preparation is free of non-specific nucleases. Bsp cleaves double-stranded DNA with the same specificity as Bacillus subtilis (Bsu) and Haemophilus aegyptius (HaeIII) restriction endonucleases, as concluded from digests and double-digests of phiX174 replicative form DNA with Bsu and Bsp. The 5'-terminal nucleotide of the cleavage products was shown to be C. Bacillus sphaericus R produces Bsp in extremely large quantities and the enzyme can be easily purified in high yield.

References

Jan 1, 1975·Annual Review of Biochemistry·D Nathans, H O Smith
Jan 5, 1975·Journal of Molecular Biology·Richard J RobertsP A Myers
Jan 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R Wu, A D Kaiser
Mar 1, 1974·Virology·P HumphriesP Connolly
Nov 1, 1967·Biochemistry·J A Reynolds, M J Schlesinger
Jul 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·C C Richardson

Citations

Mar 31, 1980·Molecular Biology Reports·I Fodor, T Beridze
Aug 1, 1979·Quarterly Reviews of Biophysics·P Modrich
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K Postle, R F Good
Mar 11, 1982·Nucleic Acids Research·X Y Zhang, W Hörz
Mar 11, 1982·Nucleic Acids Research·Richard J Roberts
Dec 21, 1984·Nucleic Acids Research·A Bernardi, F Bernardi
Apr 25, 1990·Nucleic Acids Research·Richard J Roberts
Jan 1, 1979·Nucleic Acids Research·J ForstováJ Doskocil
Jan 11, 1980·Nucleic Acids Research·Richard J Roberts
Oct 24, 1980·Nucleic Acids Research·W Pfeiffer, H G Zachau
Jun 15, 1983·European Journal of Biochemistry·I G PashevG G Markov
Jan 24, 1983·FEBS Letters·A A JanulaitisB Jaskeleviciene
Oct 15, 1979·Analytical Biochemistry·K Baksi, G W Rushizky
Jan 1, 1982·CRC Critical Reviews in Biochemistry·P Modrich
Sep 1, 1981·Analytical Biochemistry·A A Janulaitis, D P Vaitkevitchius
Sep 1, 1978·European Journal of Biochemistry·C KonczP Venetianer

Related Concepts

DNA Sequence
DNA Restriction Enzymes
DNA, Bacterial
Substrate Specificity

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