A new signal-on method for the detection of protein based on binding-induced strategy and photoinduced electron transfer between Ag nanoclusters and split G-quadruplex-hemin complexes

Analytica Chimica Acta
Kai ZhangMinhao Xie

Abstract

Proteins play important roles in biological and cellular processes. The levels of proteins can be useful biomarkers for cellular events or disease diagnosis, thus the method for sensitive and selective detection of proteins is imperative to proteins express, study, and clinical diagnosis. Herein, we report a "signal-on" platform for the assay of protein based on binding-induced strategy and photoinduced electron transfer between Ag nanoclusters and split G-quadruplex-hemin complexes. By using biotin as the affinity ligand, this simple protocol could sensitively detect streptavidin with a detection limit down to 10 pM. With the use of an antibody as the affinity ligand, a method for homogeneous fluorescence detection of Prostate Specific Antigen (PSA) was also proposed with a detection limit of 10 pM. The one-step and wash-free assay showed good selectivity. Its high sensitivity, acceptable accuracy, and satisfactory versatility of analytes led to various applications in bioanalysis.

References

Sep 8, 2005·Journal of the American Chemical Society·Chao LiChongwu Zhou
Jan 14, 2012·Analytical Chemistry·Hongquan ZhangX Chris Le
Dec 13, 2012·Chemical Reviews·Hongquan ZhangX Chris Le
Jan 31, 2013·Journal of the American Chemical Society·Feng LiX Chris Le
Jul 3, 2013·Chemical Reviews·Shengwen ShenX Chris Le
Mar 20, 2014·Nanotechnology·Sylwia WalczakJuewen Liu
Jun 6, 2014·Chemical Communications : Chem Comm·Zhiqin YuanHuan-Tsung Chang
Jun 17, 2014·Chemical Reviews·Wei-Wei ZhaoHong-Yuan Chen
Aug 26, 2014·Biosensors & Bioelectronics·Hee Dong JangJeong-Woo Choi

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