A new site-specific endonuclease from Streptomyces lavendulae (SlaI)

Gene
H TakahashiH Sugisaki

Abstract

A new restriction-like endonuclease, SlaI, was found and partially purified from Streptomyces lavendulae ATCC8664. This endonuclease cleaved bacteriophage lambda DNA at only one site, and cytosine-substituted bacteriophage T4 DNA at 16 sites. The recognition sequence was determined by using SlaI fragments of cytosine-substituted bacteriophage T4 DNA. The hexanucleotide recognized by SlaI endonuclease was (Formula: see text), with the sites of cleavage as indicated by the arrows. Therefore, SlaI endonuclease was an isoschizomer of XhoI endonuclease.

References

Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M ThomasR W Davis
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A Rambach, P Tiollais
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·F SangerD Fischer
Dec 1, 1969·European Journal of Biochemistry·G G Brownlee, F Sanger

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Citations

Mar 11, 1982·Nucleic Acids Research·R J Roberts
Apr 25, 1990·Nucleic Acids Research·R J Roberts
Jan 11, 1980·Nucleic Acids Research·R J Roberts
Jan 10, 1981·Nucleic Acids Research·R J Roberts
Feb 1, 1996·Canadian Journal of Microbiology·I S NovellaJ Sánchez
Jul 15, 1982·Virology·H Takahashi, H Saito
May 6, 2003·Annual Review of Phytopathology·Brion DuffyJos M Raaijmakers

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