A new solid phase extraction clean-up method for the determination of 12 type A and B trichothecenes in cereals and cereal-based food by LC-MS/MS

Molecular Nutrition & Food Research
Marianna KlötzelHans-Ulrich Humpf

Abstract

A new reliable and cost-efficient solid phase extraction-based clean-up method for the determination of 12 type A and B trichothecenes [deoxynivalenol (DON), nivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, fusarenon-X, T-2 toxin, HT-2 toxin, neosolaniol, monoacetoxy-scirpenol, diacetoxyscirpenol, T-2 triol and T-2 tetraol] in cereals and cereal-based food is presented. Furthermore, the suitability for the simultaneous determination of zearalenone is examined. Toxins were extracted from cereal samples using ACN/water (80/20, v/v), purified by means of a new Bond Elut Mycotoxin column and analyzed via liquid chromatography-electrospray ionization tandem mass spectrometry. Limits of detection were calculated for the matrix wheat and ranged from 0.3 to 5 ng/g, depending on the toxin. Average recovery rates for the tested compounds in seven cereal-based matrices have been determined ranging from 65 to 104%. The relative standard deviations of the complete method ranged from 2.67 (DON, wheat) to 20.0% (T-2 toxin, oats).

References

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Nov 10, 2005·Journal of Agricultural and Food Chemistry·Marianna KlötzelHans-Ulrich Humpf
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Citations

Mar 1, 2011·Food Additives & Contaminants. Part B, Surveillance·L González-Osnaya, A Farrés
Jun 1, 2011·Food Additives & Contaminants. Part B, Surveillance·Hyun Ee OkHyang Sook Chun
Oct 13, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·P G JinY P Ren
Oct 31, 2015·Analytical and Bioanalytical Chemistry·Katharina Habler, Michael Rychlik
Apr 7, 2007·International Journal of Food Microbiology·Timothy O AdejumoPetr Karlovsky
Apr 15, 2014·International Journal of Food Microbiology·L K NielsenR V Ray
Sep 26, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Yamei TangKeping Shen
Apr 22, 2009·The Analyst·Priscilla BowensHanspeter Naegeli
Mar 16, 2013·International Journal of Molecular Sciences·Dima AlkadriAnnamaria Pisi
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Mar 8, 2006·Molecular Nutrition & Food Research·Michael BretzHans-Ulrich Humpf
Feb 10, 2009·Journal of Agricultural and Food Chemistry·Marita BeyerHans-Ulrich Humpf
Jul 25, 2021·International Journal of Molecular Sciences·Jakub PastuszakAgnieszka Płażek
Jul 14, 2021·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Diego Jorquera-PereiraGisela Ríos-Gajardo
Sep 21, 2007·Journal of Agricultural and Food Chemistry·Benedikt CramerHans-Ulrich Humpf
Aug 17, 2006·Journal of Agricultural and Food Chemistry·Michael BretzHans-Ulrich Humpf

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