PMID: 6159321Jan 1, 1980Paper

A new surface antigen (PC.2) expressed exclusively on plasma cells

Immunogenetics
N TadaU Hämmerling

Abstract

Somatic cell hybridization of NS.1 nonsecretor myeloma cells with spleen cells of (DBA/2 X C57BL/6)F1 mice immunized against the myeloma MOPC 70A of BALB/c mice led to the establishment of five hybridoma clones which continuously secrete anti-MOPC 70A cytotoxic antibodies. The respective antigen detected by each of the five monoclonal antibodies is expressed both on plasmacytomas and on antibody-secreting cells as the only normal cell type. The tissue distribution of this new antigen is different from that reported for the alloantigen PC.1, and we have therefore designated it as PC.2. On the basis of immune elimination of direct and indirect plaque-forming cells, all mouse strains tested express PC.2 determinants, identifying PC.2 essentially as an autoantigen. Conventional anti-PC.1 alloantiserum contains antibodies to the PC.2 determinant, and these antibodies are distinguishable from the anti-PC.1 antibodies proper by the fact that only the latter are absorbed by liver cells. Monoclonal anti-PC.2 antibodies are not directed against MuLV-(murine leukemia virus)--associated antigens as over 20 ecotropic, several MCF (mink colony forming recombinant, and xenotropic viruses failed to react in immunofluorescence assays.

References

Dec 1, 1971·European Journal of Immunology·T TakahashiE A Boyse
Mar 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M Cikes, S Friberg
Jun 1, 1970·The Journal of Experimental Medicine·T TakahashiE A Boyse
Sep 1, 1967·The Journal of Experimental Medicine·R I Mishell, R W Dutton

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