A new TAG-72 cancer marker peptide identified by phage display.

Cancer Letters
Ling ChenM Rusckowski

Abstract

Radiolabeled peptides as markers of cancer targets have demonstrated their value in diagnostic imaging and radiotherapy. The 16 mer f88-4/Cys6 phage display library was applied to affinity purified TAG-72 and three consensus peptides were identified: VHHSCTKLTHCCQNWH (A2-13), GGVSCMQTSPVCENNL (A2-6) and TKRDCSAQNYGCQKAI (A2-11). The A2-13 and A2-6 phages showed the highest percent binding to LS-174T cells by flow cytometry and were 3-fold higher than a control phage, while fluorescence microscopy showed that both A2-6 and A2-13 phages bound to the LS-174T cell membrane. However, only the A2-6 phage demonstrated specificity by low binding to the TAG-72 negative cell HT-29. Furthermore, the synthesized free A2-6 peptide demonstrated specific binding to LS-174T cells by flow cytometry and by immunohistochemical staining of xenograft tumor compared to normal colon. These data indicate that the A2-6 peptide is specific for the TAG-72 cancer target.

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Citations

Mar 22, 2012·Pathology Oncology Research : POR·Yang ZhangYang-de Zhang
Dec 5, 2013·Chemical Reviews·Bethany Powell Gray, Kathlynn C Brown
Aug 31, 2011·Nuclear Medicine Communications·Ling ChenMary Rusckowski
Jan 26, 2016·Molecular and Cellular Probes·Juanjuan HanYingchun Hou
May 25, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Lan LuChi Hin Cho
Oct 10, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Chuying MaZhongbing Huang
Mar 7, 2013·Cancer Letters·Carolina Fernandes ReisLaura Sterian Ward
Oct 29, 2010·Cancer Biology & Therapy·Nan XiaoMary Rusckowski
May 24, 2016·Advanced Drug Delivery Reviews·Ruiwu LiuKit S Lam
May 17, 2017·Advanced Drug Delivery Reviews·Ayelet David
Feb 21, 2018·Advanced Materials·Guo-Bin QiHao Wang
Jan 17, 2013·British Journal of Cancer·J P TiernanT A Hughes
Nov 20, 2012·Oncology Letters·Yun-Peng ZhangWu-Li Yang

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