A new technique for quantitative determination of dexamethasone in pharmaceutical and biological samples using kinetic spectrophotometric method

International Journal of Analytical Chemistry
Ali Mohammad Akhoundi-Khalafi, Masoud Reza Shishehbore

Abstract

Dexamethasone is a type of steroidal medications that is prescribed in many cases. In this study, a new reaction system using kinetic spectrophotometric method for quantitative determination of dexamethasone is proposed. The method is based on the catalytic effect of dexamethasone on the oxidation of Orange G by bromate in acidic media. The change in absorbance as a criterion of the oxidation reaction progress was followed spectrophotometrically. To obtain the maximum sensitivity, the effective reaction variables were optimized. Under optimized experimental conditions, calibration graph was linear over the range 0.2-54.0 mg L(-1). The calculated detection limit (3s b/m) was 0.14 mg L(-1) for six replicate determinations of blank signal. The interfering effect of various species was also investigated. The present method was successfully applied for the determination of dexamethasone in pharmaceutical and biological samples satisfactorily.

References

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Mar 29, 2001·Obstetrics and Gynecology·S L BloomK J Leveno
Nov 30, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mei ZhangPhilip A Bird

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Citations

Mar 30, 2017·Journal of Analytical Methods in Chemistry·César SotoM Inés Toral

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Methods Mentioned

BETA
electrophoresis

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