A new technology for mutation detection

Annals of the New York Academy of Sciences
William H ShackelfordJ Wang

Abstract

A sensitive, accurate, and simple method, called shifted termination assay (STA), was developed for detection of genetic mutations. The STA technology can be used to detect genetic mutations in polymerase chain reaction (PCR)-amplified samples of tissue, and plasma and serum that include circulating DNA containing point mutations, insertions, deletions, translocations, or single nucleotide polymorphisms (SNPs). STA is a multiple-base and multiple-cycle primer extension based detection method that can identify mutant DNA in samples containing as little as 1% mutant DNA in a mixture with 99% wild-type DNA. On the basis of the STA method, a mutation detection kit, called Mutector, was developed. Mutector is a 96-well microplate format test kit with colorimetric or luminescent detection options. An initial study shows that Mutector is more accurate and sensitive than sequencing and PCR restriction enzyme analysis methods in detecting DNA mutations from clinical samples.

References

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Citations

Aug 26, 2006·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Long JinRicardo V Lloyd
Dec 7, 2010·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Peter W SchlosshauerJianli Dong
Oct 12, 2012·The Journal of Clinical Endocrinology and Metabolism·Carol LiMartha A Zeiger
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May 30, 2007·Biochemical and Biophysical Research Communications·Takanobu YoshimotoHajime Kubo
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Oct 17, 2009·Clinical Chemistry·Rajyalakshmi Luthra, Zhuang Zuo
Jun 14, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David S HongAntonio Jimeno

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