A new temperature-insensitive allele of the Arabidopsis AXR6/CUL1 locus derived from a missense mutation in the C-terminal RBX1 binding region

Plant Signaling & Behavior
Yukiko MoriKotaro T Yamamoto

Abstract

We isolated a new recessive allele at the AUXIN RESISTANT6/CULLIN1 (AXR6/CUL1) locus, axr6-101, from an EMS-mutagenized population of Arabidopsis thaliana, the Landsberg erecta ecotype. axr6-101 is auxin resistant and semi-dwarf similar to the other recessive axr6 mutants. The axr6-101 phenotype is caused by the E716K substitution of the CUL1 protein, which is likely to affect its ability to bind to the C-terminal RING domain of RING-box 1 (RBX1). The previously reported allele of AXR6, cul1-7, is caused by a substitution at T510 that binds to the N-terminal β-strand of RBX1. Although cul1-7 shows temperature-sensitive phenotype, the axr6-101 phenotype is largely unaffected by temperature. axr6-101 may provide an important genetic resource for study of the structure-function relationship of the CUL1 protein.

References

Apr 19, 2002·Nature·Ning ZhengNikola P Pavletich
Jun 12, 2002·Molecular Biology of the Cell·Wen-Hui ShenPascal Genschik
Jun 18, 2002·Plant Physiology·Georg JanderRobert L Last
May 27, 2005·Nature·Nihal DharmasiriMark Estelle
Jul 28, 2005·The Plant Journal : for Cell and Molecular Biology·Marcel QuintWilliam M Gray
Dec 13, 2006·Plant Physiology·Jennifer MoonMark Estelle
Feb 4, 2011·Current Opinion in Structural Biology·David M DudaBrenda A Schulman

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BETA
neddylation

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