A new test based on 'salting out' to measure relative surface hydrophobicity of bacterial cells
A simple method for quantification of the hydrophobic surface properties of bacteria is described. The method is based on precipitation of cells by salts, for instance (NH4)2SO4. The order in which cells are precipitated is a measure of their surface hydrophobicities, the most hydrophobic cells being first precipitated at low salt concentration. Temperature, pH, time and the bacterial cell concentration were shown to affect the results. When these variables were kept constant the method was highly reproducible. This 'salting out' method was applied to enterotoxigenic Escherichia coli strains with different surface protein antigens (fimbriae, fibrillae and colonization factor antigen, CFA). These enterotoxigenic E. coli strains were found to have surface hydrophobicity in the following order: CFA/I greater than CFA/II greater than K88 similar to K99 greater than type 1.
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Surface hydrophobicity of "rheumatogenic" and "nephritogenic" strains of group A streptococci and the ultrastructural surface feature of pharyngeal cells exposed to group A streptococci
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The effects of three non-antibiotic, antimicrobial agents on the surface hydrophobicity of certain micro-organisms evaluated by different methods
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Cell surface hydrophobicity of Actinomyces pyogenes determined by hexadecane adherence- and salt aggregation studies
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Involvement of a sialic acid-binding lectin with hemagglutination and hydrophobicity of Flavobacterium psychrophilum
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Adhesive and hydrophobic properties of Escherichia coli from the rectal mucosa of patients with ulcerative colitis
Bacterial cell surface hydrophobicity properties in the mediation of in vitro adhesion by the rabbit enteric pathogen Escherichia coli strain RDEC-1
Characterization of the cell surface properties of drinking water pathogens by microbial adhesion to hydrocarbon and electrophoretic mobility measurements
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Variability in biofilm formation correlates with hydrophobicity and quorum sensing among Vibrio parahaemolyticus isolates from food contact surfaces and the distribution of the genes involved in biofilm formation
Characterization and molecular cloning of the PCF8775 CS5 antigen from an enterotoxigenic Escherichia coli 0115:H40 isolated in Central Australia
Inhibition of resistance and virulence in itraconazole-resistant Candida albicans by butylated hydroxyanisole
Tetracycline in combination with sodium dioctylsulfosuccinate show increased antimicrobial activity in resistant microorganisms
Effects of subinhibitory concentrations of ciprofloxacin on enterotoxigenic Escherichia coli virulence factors
Surface properties of Streptococcus phocae strains isolated from diseased Atlantic salmon, Salmo salar L
Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms
Sequential hydrophobic partitioning of cells of Pseudomonas aeruginosa gives rise to variants of increasing cell-surface hydrophobicity
Surface hydrophobicity of petroleum hydrocarbon degrading Burkholderia strains and their interactions with NAPLs and surfaces
A comparison of various methods to predict bacterial predilection for organic solvents used as reaction media
Characterization by high-performance liquid chromatography with diode-array detection coupled to time-of-flight mass spectrometry of the phenolic fraction in a cranberry syrup used to prevent urinary tract diseases, together with a study of its antibacterial activity
Study of the impact of cranberry extract on the virulence factors and biofilm formation by Enterococcus faecalis strains isolated from urinary tract infections
Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation
Hydrophobic features of EPS extracted from anaerobic granular sludge: an investigation based on DAX-8 resin fractionation and size exclusion chromatography
Standardization of salt aggregation test for reproducible determination of cell-surface hydrophobicity with special reference to Staphylococcus species
Cell surface hydrophobicity and charge of Staphylococcus aureus and coagulase-negative staphylococci from bovine mastitis
Evaluation of hydrophobicity and adherence of Neisseria meningitidis strains and a study of their correlation by analysis of alterations induced by antibiotics
Capsulated cells of Aeromonas salmonicida grown in vitro have different functional properties than capsulated cells grown in vivo
Antibacterial activity of isolated phenolic compounds from cranberry (Vaccinium macrocarpon) against Escherichia coli
Comparison of seroagglutination, ELISA, and indirect fluorescent antibody staining for the detection of K99, K88, and 987P pilus antigens of Escherichia coli
Adherence of coagulase-negative staphylococci onto polyethylene catheters in vitro and in vivo: a study on the influence of various plasma proteins
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Role of the metalloprotease Vvp and the virulence plasmid pR99 of Vibrio vulnificus serovar E in surface colonization and fish virulence
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Cell surface hydrophobicity of Candida albicans isolated from elder patients undergoing denture-related candidosis
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Evidence for Escherichia coli O157:H7 attachment to water distribution pipe materials by scanning electron microscopy
Bacterial hydrophobicity measured as partition of palmitic acid between the two immiscible phases of cell surface and buffer
Effect of 2,4-dichlorophenoxyacetic acid herbicide on escherichia coli growth, chemical composition, and cellular envelope
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