A new Tetrahymena actin-binding protein is localized in the division furrow

Journal of Biochemistry
A WatanabeO Numata

Abstract

Using an F-actin affinity column, a 60 kDa fragment of a 71 kDa F-actin-binding protein was partially purified from Tetrahymena pyriformis. After digestion of the 60 kDa fragment with cyanogen bromide, the N-terminal 21-amino acid sequence of one of the resulting peptides was found to show sequence similarity to a region near the actin-binding site (amino acid residues 260-281) of yeast fimbrin. An antibody prepared against a synthesized 21-mer oligopeptide reacted with the 71 kDa proteins in T. pyriformis and T. thermophila cell extracts, suggesting that the 60 kDa fragment was produced from the 71 kDa protein through partial digestion occurring during isolation. The 60 kDa fragment bound to Tetrahymena F-actin as well as to rabbit skeletal muscle F-actin, and induced the bundling of Tetrahymena F-actin. Indirect immunofluorescence revealed colocalization of the 71 kDa protein and actin in the oral apparatus and the deep fiber bundles in T. pyriformis. On the other hand, in T. thermophila, the 71 kDa protein was localized in the oral apparatus and the contractile vacuole pores during the interphase. During cytokinesis, the 71 kDa protein was localized in the division furrow. Therefore, the 71 kDa protein seems to associate wit...Continue Reading

Citations

Apr 25, 2000·Cell Biology International·O NumataK Gonda
Feb 21, 2002·Molecular Biology of the Cell·Edward HarrisJames Cardelli
Jun 18, 2005·Acta Pharmacologica Sinica·Veerle DelanoteJan Gettemans
Apr 11, 2002·Cell Structure and Function·O Numata, K Gonda
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
May 18, 2000·Microscopy Research and Technique·O NumataY Kurasawa
May 22, 2019·Biomolecules·Joana LeiteAna Xavier Carvalho
Jan 22, 2004·The Journal of Eukaryotic Microbiology·Roland E HoseinR H Gavin
Apr 12, 2002·Biochemical and Biophysical Research Communications·Kohsuke Gonda, Osamu Numata
Oct 21, 1999·Biochemical and Biophysical Research Communications·K GondaO Numata

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