A new tissue-slicing method for the study of function and position of somatotrophs contained within the male rat pituitary gland

Endocrinology
F M Perez, W C Hymer

Abstract

A tissue-slicing method has been developed for obtaining quantitative information relative to the function and distribution of somatotrophs in the individual pituitary of the male rat. The adenohypophysis was cut along midtransverse, coronal, and sagittal planes, dividing the gland into eight distinct slices. Basal (unstimulated) GH release was measured from sections placed in gyrotory incubation for three consecutive periods (20 min each). Response to rat hypothalamic GH-releasing hormone (rhGHRH) was studied in slices placed in perifusion and in dispersed cells placed in static incubation. Tissues and cells were analyzed by light and electron microscopy and flow cytometry. Cells retained good morphological integrity and viability (greater than 85%) after incubation (1 h) or perifusion (5 h). Somatotrophs were evenly distributed sagittally and rostrocaudally, but were unevenly distributed dorsoventrally; mean values for dorsal and ventral sections were 45.3 +/- 2.5% and 38.9 +/- 1.9%, respectively. Differential basal GH release was observed after the first gyrotory incubation period; values differed by as much as 3-fold. These differences became less apparent with subsequent incubations. Pituitary slices in perifusion showed a...Continue Reading

Citations

Jan 1, 1994·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·W C HymerR Grindeland
Apr 1, 1991·Molecular and Cellular Endocrinology·P MukherjeeW C Hymer
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Nov 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·R RameshW J Kuenzel
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Dec 7, 2019·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Wesley C HymerWilliam J Kraemer

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