A new tool for detection of extracellular traps

Methods and Applications in Fluorescence
Alizé ProustMichel J Tremblay

Abstract

Extracellular traps ejected by various immune cells (neutrophils, macrophages, eosinophils and mast cells) have several immune functions, either protective against pathogens or deleterious in some autoimmune or inflammatory disorders. Since their first description in 2004, the mechanisms of extracellular traps formation have been extensively investigated though still not fully understood. We describe here a new tool for the detection of extracellular traps by fluorescence microscopy in a single-step staining protocol, which does not require any wash. The approach uses the GreenGlo™ DNA dye, which can differentiate between nuclear DNA and extracellular DNA (extracellular traps) released from cells using different fluorescence excitation wavelengths. GreenGlo™ staining is suitable for adherent and non-adherent cells and is expected to be extendable to extracellular traps from other cells types (i.e. eosinophils, mast cells and monocytes).

References

Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Sep 8, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mariana J Kaplan, Marko Radic
Feb 20, 2014·Infection and Immunity·Marc RöhmConstantin F Urban
Dec 30, 2014·Immunology·Oscar Rodríguez-EspinosaFrancisco Javier Sánchez-García
Apr 17, 2015·Journal of Leukocyte Biology·Devin M BoeElizabeth J Kovacs
Jun 14, 2016·Journal of Immunology Research·Nicole de Buhr, Maren von Köckritz-Blickwede
Oct 11, 2017·Nature Reviews. Immunology·Venizelos Papayannopoulos

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Citations

May 24, 2021·Clinical Reviews in Allergy & Immunology·Daniel Elieh Ali Komi, Wolfgang M Kuebler

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