A new transgenic reporter line reveals Wnt-dependent Snai2 re-expression and cranial neural crest differentiation in Xenopus

Scientific Reports
Jiejing LiShuo Wei

Abstract

During vertebrate embryogenesis, the cranial neural crest (CNC) forms at the neural plate border and subsequently migrates and differentiates into many types of cells. The transcription factor Snai2, which is induced by canonical Wnt signaling to be expressed in the early CNC, is pivotal for CNC induction and migration in Xenopus. However, snai2 expression is silenced during CNC migration, and its roles at later developmental stages remain unclear. We generated a transgenic X. tropicalis line that expresses enhanced green fluorescent protein (eGFP) driven by the snai2 promoter/enhancer, and observed eGFP expression not only in the pre-migratory and migrating CNC, but also the differentiating CNC. This transgenic line can be used directly to detect deficiencies in CNC development at various stages, including subtle perturbation of CNC differentiation. In situ hybridization and immunohistochemistry confirm that Snai2 is re-expressed in the differentiating CNC. Using a separate transgenic Wnt reporter line, we show that canonical Wnt signaling is also active in the differentiating CNC. Blocking Wnt signaling shortly after CNC migration causes reduced snai2 expression and impaired differentiation of CNC-derived head cartilage struc...Continue Reading

References

Aug 24, 1999·Developmental Biology·T F CarlM W Klymkowsky
Dec 28, 1999·Protein Engineering·P Corish, C Tyler-Smith
Dec 11, 2002·The Journal of Cell Biology·Lisette HariLukas Sommer
Dec 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Annamaria LocascioM Angela Nieto
Dec 24, 2002·Trends in Neurosciences·Jinling WuPeter S Klein
Jul 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yuko Mori-AkiyamaBenoit de Crombrugghe
Jun 28, 2005·Biochemical and Biophysical Research Communications·Fumiko YanoUng-Il Chung
Sep 2, 2005·Molecular Biology of the Cell·Lisa A Taneyhill, Marianne Bronner-Fraser
Jan 18, 2006·Mechanisms of Development·Hajime OginoRobert M Grainger
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stephen A Murray, Thomas Gridley
Oct 27, 2006·Development·Thomas J CarneyRobert N Kelsh
Jan 6, 2007·PloS One·Chi ZhangMichael W Klymkowsky
Mar 23, 2007·Development·Stephen A MurrayThomas Gridley
May 10, 2007·Nature Protocols·Hajime OginoRobert M Grainger
Sep 18, 2009·Nature·Shih-Min A HuangFeng Cong
Sep 2, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hong Thi TranKris Vleminckx
Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Perrine BarraudClare V H Baker
Feb 11, 2011·Genesis : the Journal of Genetics and Development·Young-Hoon Lee, Jean-Pierre Saint-Jeannet
Jul 1, 2011·Development·Jianli ShiMichael W Klymkowsky
Jan 10, 2012·Developmental Biology·Shuo WeiDouglas W DeSimone
Apr 24, 2012·Genesis : the Journal of Genetics and Development·Frederico S L M RodriguesRobert N Kelsh
May 2, 2012·Cellular and Molecular Life Sciences : CMLS·Timothy J Stuhlmiller, Martín I García-Castro
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhao-Qiu WuStephen J Weiss
Nov 23, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Young-Hoon LeeJean-Pierre Saint-Jeannet
Feb 5, 2013·Cold Spring Harbor Perspectives in Biology·Shachi BhattPaul A Trainor
May 16, 2013·Development·Roberto Mayor, Eric Theveneau
Jan 8, 2015·Development·Marcos Simões-Costa, Marianne E Bronner
Mar 24, 2015·PloS One·Hiroshi KurosakaAngelo Iulianella
Apr 30, 2015·Development·Elias H BarrigaRoberto Mayor
Feb 18, 2016·Developmental Biology·Chaolie HuangJubin Kashef
Mar 13, 2016·Current Topics in Developmental Biology·Paul A Trainor

❮ Previous
Next ❯

Citations

Oct 2, 2019·Cells·Yu JiChengji J Zhou
Dec 16, 2020·Development·Mark PerfettoShuo Wei
May 29, 2021·Developmental Biology·Jorge B AquinoLaura A Montaldo

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
RNA-seq
whole-genome
PCR

Software Mentioned

ZEN

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.