A new type of antiserum to thyrotropin-releasing hormone; detection of new TRH-immunoreactive cell groups in rat hypothalamus

Experientia
T AnrakuM Shirouzu

Abstract

We produced a new type of antiserum to thyrotropin-releasing hormone (TRH) in rabbits. The immunogen is TRH-BSA, the production of which is based on the formation of an amide bond using carbodiimide (EDC). The specificity of the antiserum was assessed by enzyme immunoassay (EIA) and immunohistochemistry. When using the anti-TRH serum for immunohistochemistry in rat hypothalamus, new magnocellular groups were detected in the ventrolateral parts of the posterior hypothalamus and the dorsal parts of the third ventricle. Colchicine treatment was found not to be necessary to visualize perikarya containing TRH.

References

Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L GuesdonS Avrameas
Mar 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G W Kreutzberg
Mar 1, 1972·Endocrinology·R M Bassiri, R D Utiger
Nov 6, 1969·Biochemical and Biophysical Research Communications·J BolerA V Schally
Nov 5, 1971·Biochimica Et Biophysica Acta·J M Fernández SousaA Ribera
Dec 1, 1968·European Journal of Pharmacology·A Dahlström
Oct 20, 1966·Biochemical and Biophysical Research Communications·A V SchallyJ F Barrett
Apr 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O Johansson, T Hökfelt
Apr 10, 1980·Nature·T HökfeltM Schultzberg
Jan 1, 1980·Experimental Brain Research·O JohanssonL A Sternberger
Sep 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y F Chen, V D Ramirez
Aug 1, 1981·The Journal of Comparative Neurology·H KimuraE G McGeer

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