Abstract
Mitochondrial DNA of the pathogenic yeast Candida albicans contains a species-specific DNA region, designated EO3, within the duplicated region in its stem-and-loop structure, as reported in our previous paper. We have revealed that the EO3 region exhibits a DNA-size polymorphism, yielding three fragment length types: L, M, and S. This polymorphism is due to the presence (or absence) of two small nucleotide sequences of ~50 bp (designated a and b) within the EO3 region: type L possesses both a and b, type M possesses a alone, and type S possesses neither a nor b. Here we have identified a new type of EO3, which possesses b but not a, designated type M-II. Restriction fragment analyses with BglII revealed accurate discrimination of EO3 into four types: L, M-I, M-II, and S. Moreover, we show that these small nucleotide segments a and b were encompassed by the characteristic nucleotide sequence with a common inverted repeat structure. In the present report we propose a revised version of EO3 typing, suggesting epidemiological usefulness as an alternative tool for species-specific detection of C. albicans, and discuss the potentiality of EO3 for generating more variations of DNA polymorphism.
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1992·Journal of Clinical Microbiology·Y MiyakawaY Fukazawa
Nov 1, 1989·Somatic Cell and Molecular Genetics·A M ChanC S Cooper
Nov 1, 1989·Journal of Bacteriology·J A ShawW S Riggsby
Oct 1, 1985·Journal of Bacteriology·J W WillsW S Riggsby
Jan 1, 1984·Antonie van Leeuwenhoek·W J MiddelhovenN J Kreger-Van Rij
Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·Y Miyakawa, T Mabuchi
Dec 1, 1993·Journal of Clinical Microbiology·Y MiyakawaY Fukazawa
Aug 2, 2000·Journal of Clinical Microbiology·R WahyuningsihP Schnitzler
Aug 3, 2000·Yeast·Y Miyakawa
Dec 2, 2000·Journal of Clinical Microbiology·F DalleA Bonnin
Feb 24, 2001·Medical Mycology·E ReissR M Zancopé-Oliveira
Jul 28, 2001·Journal of Clinical Microbiology·C FerrerJ L Alió
Sep 28, 2001·Journal of Clinical Microbiology·H C ChangT C Chang
Jul 5, 2002·Clinical Microbiology Reviews·Siew Fah Yeo, Brian Wong
Dec 10, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·François StéphanStéphane Bretagne
Apr 26, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hilmar WisplinghoffMichael B Edmond
Nov 5, 2003·Diagnostic Microbiology and Infectious Disease·Todd M PryceChristopher H Heath
Aug 6, 2005·Journal of Clinical Microbiology·Paula SampaioCélia Pais
Nov 15, 2006·BMC Infectious Diseases·Odile EloyStéphane Bretagne
Jan 16, 2007·Clinical Microbiology Reviews·M A Pfaller, D J Diekema
Apr 10, 2007·Eukaryotic Cell·Frank C OddsChristophe d'Enfert
Oct 12, 2007·Journal of Clinical Microbiology·Dea Garcia-HermosoStéphane Bretagne