A new type of transporter with a new type of cellular function: L-lysine export from Corynebacterium glutamicum

Molecular Microbiology
M VrljicL Eggeling

Abstract

We discovered that after deregulation of the L-lysine biosynthesis in Corynebacterium glutamicum, L-lysine accumulated in the cytosol and the efflux properties of this amino acid in mutants used for L-lysine production were altered. In this study we describe the cloning and molecular identification of lysE, which encodes the translocator specifically exporting L-lysine from the cell. The lysE gene product does not display homology to any known transporter. It is only 236 amino acids in size, with the potential to span the membrane six times. The LysE protein was oversynthesized to confirm its deduced M(r) of 25425 Da. A probable regulatory gene, lysG, is localized immediately adjacent to lysE and displays all the typical structural features of an autoregulatory transcriptional regulator of the LysR-type family. L-Lysine export is correlated with lysE expression. A null mutant is unable to excrete L-lysine, whereas with overexpressed lysE, L-lysine is exported at a rate of 3.76 nmol min-1 mg-1 dry weight, which is five times the rate that was obtained with the wild type. A deletion mutant was constructed to search for a natural function of this unique carrier. Surprisingly, growth of this mutant is abolished on a salt medium in ...Continue Reading

Citations

Oct 6, 2006·Applied Microbiology and Biotechnology·Masaru Wada, Hiroshi Takagi
Jul 28, 2010·Applied Microbiology and Biotechnology·Jens Schneider, Volker F Wendisch
May 10, 2011·Applied Microbiology and Biotechnology·Jens Schneider, Volker F Wendisch
Jan 13, 2006·Journal of Industrial Microbiology & Biotechnology·Arnold L Demain
Mar 10, 2001·Chemosphere·M Kircher, W Pfefferle
Jun 5, 2003·Journal of Microbiological Methods·Marian C J BlokpoelHuw D Williams
Feb 14, 2003·FEMS Microbiology Letters·Anne W GrantElizabeth M Ellis
May 14, 1999·Trends in Biochemical Sciences·V V AleshinV A Livshits
Jul 4, 2001·European Journal of Biochemistry·D L JackM H Saier
May 17, 2007·Genes & Development·Rakesh S Laishram, Jayaraman Gowrishankar
May 3, 2011·Applied and Environmental Microbiology·Hatsuhiro HoriRyoichi Katsumata
Apr 25, 2001·Applied and Environmental Microbiology·A LoosA J Sinskey
Apr 27, 2002·Applied and Environmental Microbiology·Eva RadmacherLothar Eggeling
Jan 25, 2011·Journal of Bacteriology·Norma StäblerLothar Eggeling
Feb 4, 2003·Journal of Bacteriology·Isabel FrankeAugust Böck
May 20, 2005·Journal of Bacteriology·Christian TrötschelReinhard Krämer
Oct 20, 2005·Journal of Bioscience and Bioengineering·Hiroshi Shimizu
Nov 15, 2000·Biological Chemistry·H SahmA A de Graaf
Sep 27, 2007·Bioscience, Biotechnology, and Biochemistry·Takashi MimitsukaKatsushige Yamada
Jul 19, 2012·Annual Review of Microbiology·Alain A VertèsHideaki Yukawa
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael T MeeHarris H Wang
Sep 18, 2014·FEMS Microbiology Reviews·Víctor de LorenzoAntoine Danchin
Jan 13, 2015·Current Opinion in Biotechnology·Lothar EggelingJan Marienhagen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.