PMID: 18631808Nov 1, 1980

A new viral RNA species in tobacco rattle virus-infected tissue

Virology
D M Bisaro, A Siegel

Abstract

Tobacco rattle virus (CAM strain)-infected tissue contains at least one unique species of virus-specific RNA in addition to the two virion RNAs. The new species (RNA-3; MW 0.5 x 10(6)) was characterized as follows: (1) Its synthesis and presence were detected by electrophoresis and fluorographic analysis of an extract of infected tissue that had been treated with actinomycin D and exposed to [3H]uridine; (2) its genesis from RNA-2 was determined by immobilization on specially treated diazotized paper and hybridization to cDNAs prepared to TRV RNAs 1 and 2; and (3) its template activity for a 30,000 MW protein was determined with the aid of a wheat germ-derived cell-free translation system.

Citations

Apr 30, 1982·Virology·D M Bisaro, A Siegel
Dec 1, 1984·Virology·C J Gabriel, G A de Zoeten

Related Concepts

DNA, Complementary
Cell-Free System
Dactinomycin
Electrophoresis
Immobilization
RNA
RNA, Viral
Virion
Virus Diseases
Present

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