A new way of producing isomalto-oligosaccharide syrup by using the transglycosylation reaction of neopullulanase.

Applied and Environmental Microbiology
T KurikiS Okada


A new way of producing isomalto-oligosaccharide syrup from starch was developed. Isomalto-oligosaccharides contain one or more alpha-(1-->6)-glucosidic linkages with or without alpha-(1-->4)-glucosidic linkages. The isomalto-oligosaccharide syrups are receiving increased attention as food additives because it is thought that they help prevent dental caries and improve human intestinal microflora, acting as a growth factor for bifidobacteria. The new system for production of isomalto-oligosaccharide syrup is based on the strong alpha-(1-->6)-transglycosylation reaction of neopullulanase. Bacillus subtilis saccharifying alpha-amylase was simultaneously used with neopullulanase to improve the yield of isomalto-oligosaccharides. The yield of isomalto-oligosaccharides was increased to more than 60%, compared with a yield of 45.0% obtained by the conventional system. To reduce the costs, the use of immobilized neopullulanase was investigated. Almost the same yield of isomalto-oligosaccharides was obtained by using immobilized neopullulanase.


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Jan 1, 1988·Microbiology and Immunology·T OoshimaS Hamada
Jan 1, 1970·Archives of Oral Biology·B Krasse, J Carlsson
Aug 20, 1988·Biotechnology and Bioengineering·T KimuraT Nakakuki

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