A new WHO International Reference Reagent for use in potency assays of inactivated poliomyelitis vaccine

Biologicals : Journal of the International Association of Biological Standardization
David J WoodE C Beuvery

Abstract

Assays of the potency of inactivated poliovirus vaccine require the use of an appropriate reference reagent. Preparation 91/574 was shown by international collaborative study to be suitable for determination of antigenic content and immunogenicity of inactivated poliovirus vaccines by in vitro and in vivo assays, respectively. The reagent is a trivalent blend of formaldehyde-inactivated monovalent pools of poliovirus type 1 (Mahoney) poliovirus type 2 (MEF-1) and poliovirus type 3 (Saukett). Studies by antigen-capture ELISA showed that the component monovalent pools contained high titres of D antigen and trace amounts of C antigen. Sucrose gradient analysis showed that the D antigenicity was almost exclusively associated with 150S virus particles. Low levels of procapsids (75S particles with D antigenicity) were detected in the type 1 and 2 monovalent pools. The profile of intact virions and procapsids in 91/574 in sucrose gradients was very similar to PU78-02, a previously used inactivated trivalent poliovirus vaccine reference. The World Health Organization (WHO) Expert Committee on Biological Standardization at its 1994 meeting established preparation 91/574 as the 2nd WHO international Reference Reagent for poliomyelitis va...Continue Reading

Citations

Sep 7, 2012·PLoS Neglected Tropical Diseases·Min-Shi LeeIh-Jen Su
Jul 12, 2011·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Mei-Liang HuangMin-Shi Lee
May 9, 2006·Biologicals : Journal of the International Association of Biological Standardization·B SimizuK Wakabayashi
May 10, 2006·Biologicals : Journal of the International Association of Biological Standardization·Maria Baca-Estrada, Elwyn Griffiths
Sep 1, 2015·Biologicals : Journal of the International Association of Biological Standardization·R ten HaveG F A Kersten
Aug 5, 2014·Vaccine·Haruko ShiratoUNKNOWN sIPV Evaluation Group of NIID-Virology II
Aug 3, 2000·International Journal of Epidemiology·L H de Oliveira, C J Struchiner
Jun 4, 2020·The Journal of Infectious Diseases·Bethany CharltonJavier Martin

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